大鼠弥漫性轴索损伤制模装置的研制及评价  

Development and evaluation of a diffuse axonal injury modeling device in rats

作　　者：王军锋[1] 宋锦宁[1] 李宇[1] 金涛[1] 刘晓斌[1] 

机构地区：[1]西安交通大学医学部第一附属医院神经外科,西安710061

出　　处：《中华神经外科杂志》2015年第11期1166-1172,共7页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金（30471774）

摘　　要：目的研制大鼠弥漫性轴索损伤（DAI）制模装置及评价制模效果。方法20只成年雄性SD大鼠随机分为对照组（5只）、DAI模型组（15只），采用头颅瞬间旋转装置制备大鼠DAI模型，以造模后6h、24h、7d为时间点分别行神经功能缺损评分（NSS），HE染色及镀银染色观察脑组织形态学的改变，免疫组织化学染色检测DAI后脑组织p淀粉样前体蛋白（B—APP）的表达。结果DAI后大鼠均表现出不同程度的昏迷，表现为无自主活动，刺痛反射、瞳孔对光反射迟钝，时间持续约50min至4h。所有大鼠清醒后活动减少，反应迟钝，不思饮食，行走不稳，各时间点NSS（6h，9．33±0．88；24h，5．67±0．67；7d，4．67±0．88）均较对照组（6h，1．33±0．67；24h，0±0；7d，0±0）明显增高（P〈0．05）。HE染色显示DAI模型组细胞数目在皮质（6h，323．33±95．82；24h，294．00±54．98；7d，277．83±52．26）、海马（7d，214．50±43．69）、脑干（6h，326．67±102．80；24h，298．33±67．56；7d，234．50±18．20）分别较对照组皮质（430．33±20．35）、海马（327．17±24．34）、脑干（429．50±81．86）明显减少（除DAI 6h模型组脑干细胞数目P〈0．05，余DAI模型亚组细胞数目P〈0．01），细胞核固缩，胶质细胞增生；在皮质及脑干神经元周围可见大量空泡及少许出血点；亦可见脑干神经纤维走行紊乱。镀银染色可见脑干神经轴索串珠样轴索球形成。免疫组化染色显示DAI模型组β—APP的表达在皮质（24h，9．16±1．32；7d，14．50±2．83）、海马（24h，10．14±2．56；7d，12．50±1．95）、脑干（6h，6．74±1．33；24h，7．64±1．11；10．09±1．77）分别较对照组皮质（4．02±0．34）、海马（4．26±0．70）、脑干（4．38±0．85）明显增高（P〈0．01），且随DAI后致伤时间的延长而增高。结论本装置能够造成大鼠脑DAI，且具有简便、�Objectives To develop a rat diffuse axonal injury （DAI） modeling device and to evaluate its molding effect. Methods A total of 20 adult male SD rats were randomly divided into either a control group （n =5） or a DAI model group （n = 15）. A rat DAI model was prepared by using a coronal rotation device. The neurological severity scores （NSS） were performed using 6 h, 24 h, and 7 d time points after modeling. HE staining and silver staining were used to observe the morphology changes of brain tissue. Immunohistochemical staining was used to detect the expression of 63-amyloid precursor protein （β-APP） in brain tissue after DAI. Results Rats showed varying degrees of coma after DAI. They showed no autonomic activities, stinging reflex, and dullness of pupillary light reflex. The coma lasted for about 50 min to 4 h. After awakening, all rats showed reduced activities, unresponsiveness, not eating, and unstable walking. NSSat each time point （6 h, 9.33±0.88, 24 h, 5.67±0.67, and7 d, 4. 67 ±0.88） was higher than that of the control group （6 h, 1.33±0.67, 24 h, 0±0, and7d, 0-±0） （P〈0.05）. HE staining showed that the number of cells in the cortex （ 6 h, 323.33± 95.82, 24 h, 294. 00 ± 54. 98, and 7 d, 277.83 ±.52.26）, hippocampus （7 d, 214.50 ±43.69） and brainstem （6 h, 326.67 ± 102.80; 24 h, 298. 33 ±67. 56; 7d, 234. 50 ± 18. 20） of the DAI model group were significantly reduced compared with that in the cortex （430. 33 ± 20. 35）, hippocampus （327. 17 ± 24. 34） , and brainstem （429. 50 ± 81.86） of the control groups （ the cell number of brainstem of the DAI 6 h model group [ P 〈 0. 05 ], the cell number other DAI model subgroups [ P 〈 0. 01 ] ）, karyopyknosis, and glial cell proliferation were found. In the cortex and around brainstem neurons, a large number of vacuolations and a few bleeding points were observed. The traveling disorders of brainstem nerve fibers were also observed. Silver staining displayed the brainstem axonal string-of-be

关 键 词：弥漫性轴索损伤 制模装置 大鼠 研制 评价 

分 类 号：R651.15[医药卫生—外科学]