同步脱氮除硫菌的筛选鉴定及其生长特性研究  

Screening,Identification and Characterization of Strains Coupling Nitrogen Removal with Sulfide Removal

作　　者：黄俊[1] 张诗颖[1] 王翻翻[1] 李月寒[1] 吴鹏[1] 宋吟玲[1,2] 

机构地区：[1]苏州科技学院环境科学与工程学院,苏州215009 [2]江苏省环境科学与工程重点实验室,苏州215009

出　　处：《生物技术通报》2015年第10期184-190,共7页Biotechnology Bulletin

基　　金：江苏省高校自然科学基金项目(13KJB610014);江苏省环境科学与工程重点实验室开放基金项目(Zd131201);江苏高校优势学科建设工程资助项目

摘　　要：为促进反硝化脱硫工艺的工程化应用,从稳定运行的反硝化脱硫UASB中筛选出2株高活性自养反硝化菌H3和H7,并对其进行了鉴定和生长及反硝化特性的研究。结果表明,2株菌均为革兰氏阴性菌,16S r DNA序列分析表明,分别与Pseudomonas stutzeri CONC12和Pseudomonas stutzeri 19smn4相似性为99.6%和98.9%。结合生理生化特性和16S r DNA序列分析,确定两菌株都为Pseudomonas stutzeri。对两菌株的生长和反硝化特性研究表明,菌株H3和H7的生长最适起始p H为6.94和6.88,最适反硝化p H分别为6.77和6.56。H3和H7的最适生长温度为30.4℃和30.6℃,最适反硝化温度分别为31.4℃和31.2℃,两株菌种都为非耐盐菌株。In order to promote the engineering application of denitrification and desulfurization process, 2 highly-active autotrophic denitrifying bacterial strains H3 and H7 were screened from stable UASB with denitrification and desulfurization, and the strains were identified and characterized. Two strains were both gram negative bacteria, the 16 S r DNA sequence analysis revealed that strain H3 and H7 had a similarity of 99.6%, 98.9% with Pseudomonas stutzeri CONC12 and P. stutzeri 19smn4 respectively. Combing the morphologic, physiobiochemical characteristics and the analysis of its 16 S r DNA, 2 strains were identified as P. stutzeri. The results of growth and denitrification of 2 strains demonstrated that the optimal initial p H for H3 and H7 growth were 6.94 and 6.88 respectively, for denitrification were 6.77 and 6.56 respectively. The optimal temperatures for growth were 30.4℃ and 30.6℃, for denitrification were 31.4℃ and 31.2℃ respectively, both of H3 and H7 were non-resistant strains to salt.

关 键 词：脱氮除硫菌 16S RDNA 反硝化特性 非耐盐菌株 

分 类 号：X703[环境科学与工程—环境工程]