阿特拉津降解群落NC1的降解特性和个体特性研究  

Transform Ability and Individual Characteristics of an Atrazinedegrading Consortium NC1

作　　者：叶金宇 李艳华 张金波[1] 高继国[1] 李海涛[1] 梁栋[1] 刘荣梅[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]哈尔滨利民农化技术有限公司,哈尔滨150525

出　　处：《农业生物技术学报》2015年第11期1509-1515,共7页Journal of Agricultural Biotechnology

基　　金：东北农业大学博士启动基金(No.2010RCB54)

摘　　要：为筛选阿特拉津高效降解菌落和菌种资源,本研究利用富集培养法,从黑龙江省伊春市南岔区长期施用阿特拉津(2-氯-4-乙胺基-6-异丙胺基-1,3,5-三嗪,Atrazine)的玉米(Zea mays L.)地中筛选出阿特拉津降解群落NC1,此群落可以在36 h内将100 mg/L阿特拉津降解为无毒的氰尿酸。通过无机盐平板直接分离法,分离出4株形态、颜色各不相同的菌株。通过生理生化和16S r DNA鉴定,菌株YJY1、YJY2、YJY3、和YJY5分别鉴定为肠道杆菌(Enterobacter sp.)、无色杆菌(Achromobacter xylosoxidans)、铜绿假单胞菌(Pseudomonas aeruginosa)和克雷白氏肺炎菌(Klebsiella pneumoniae)。其中菌株YJY5可以以阿特拉津为唯一氮源生长。降解研究表明,该群落具有良好的阿特拉津降解效果,而且群落各成员间协同作用更接近于自然界阿特拉津的降解方式。本研究结果对阿特拉津污染的生物修复工作提供参考和借鉴意义。In order to select atrazine-degrading consortium and strain resources, in the present research, by enrichment culture, a stable 4-member bacterial consortium NC1 was isolated from long-term use of atrazine surface corn-planted soil（Nancha area, Yichun City, Heilongjiang Province, China）. The consortium NC1 was attenuated and plated on mineral salts medium agar plates, strains were repeatedly crossed vaccination on mineral salts medium agar plates for separation of pure culture, 4 bacterial isolates, named strain YJY1, YJY2,YJY3 and YJY5. Based on physiological and biochemical tests, and 16 S r DNA gene sequence analysis, 4bacterial strains were shown to belong to the Gram- negative species Enterobacter sp., Achromobacter xylosoxidans, Pseudomonas aeruginosa and Klebsiella pneumonia, respectively. These species were not stated previously as being capable of atrazine- degrading. By growth capacity measuring, consortium NC1, strain YJY5 could use atrazine as single nitrogen source for growth, and strain YJY1, YJY2 and YJY3 could not.Consortium NC1 and 4 strains could hardly grow in MSM with cyanuric acid as the sole nitrogen source.Consortium NC1 and strain YJY5 had a short lag period（8-10 h）, then was logarithmic phase. Strain YJY5 was stagnate phase of 28-36 h, and consortium NC1 was still growing rapidly at 36 h and capable of degrading 100 mg/L atrazine to nontoxic cyanuric acid. Altogether this was a new combination of isolates in an atrazine- degrading consortium, and its degradation rate and the growth were both faster than that of thesole strain. The high atrazine degradation ability of the consortium NC1 showed good potential for atrazine biodegradation. This research will contribute toward a better understanding of the metabolic activities of atrazine-degrading consortium, which are generally considered to be responsible for atrazine mineralization in the natural environment. The results provide information and reference for the study on biodegradation of atrazine work.

关 键 词：阿特拉津 降解 鉴定 群落 

分 类 号：TQ638[化学工程—精细化工]