高效液相色谱-串联质谱法对城市污水A2/O处理过程中双酚a的评价  

Evaluation on Bisphenol A in A2/O Process of City Sewage Treatment using High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：孙蓉[1] 孙永琪[2] 孙峰[1] 朱逸晖 

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]扬州大学广陵学院,江苏扬州225001 [3]扬州市环境监测中心站,江苏扬州225001

出　　处：《农业灾害研究》2015年第10期80-82,共3页Journal of Agricultural Catastrophology

基　　金：扬州市环保基金(YHK1205)

摘　　要：[目的]应用高效液相色谱（HPLC）-串联质谱法（MS）测定城市污水A2／O工艺处理中双酚a的含量。[方法]样中双酚a采用c18回相萃取柱（SPE）富集，HPLC—MS分析方法，流动相为乙腈，流速为0．8ml／min，进样体积10山，相同条件下检测精密度2．5％～6．6％，标准品回收率91．5％～105．1％，方法最低检出限0．1．g／L。[结果]扬州市污水处理厂不同处理工艺过程的水样中均检出双酚a，水样质量浓度为98．3～106．5ng／L。经0ne-wavANOVA检验．F=1．258，P=0．237〉0．05。[结论]A2／0不同工艺流程对于环境雌激素处理虽然有某种下降趋势，差异无统计学意义，表明该工艺过程对于环境雌激素的降低和消除没有明显效果。[Objective]A wide variety of analytical methodologies for the analysis of bisphenol A in sewage waters have been developed in recent years. In this paper we utilized high performance liquid chromatography （I-IPLC） and mass spectrometry （MS） to measure bisphenol A level in the urban sewage waters, that have been treated with Anaerobic-Anoxic-Oxie （A2/O） technology. [Method]The bisphenol A in the water sample was enriched using Solid Phase Extraction Cleanert 18 （SPE, C18）, followed by HPLC and MS analysis. The flow rate was set at 0.8 ml/min, with aeetonitrile as the mobile phase; injection volume was 10 μleach, with a detection preci- sion of 2.5%-6.6%, and a standard recovery rate of 91.5%-105.1%. Quantitation limit of this method was 0.1 ng/L. [Results]Our analysis showed the presence of bisphenol A of all water samples that underwent different treatments from Yangzhou Sewage Treatment Plant, ranging from 98.3-106.5 ng/L. The statistical test by one-way ANOVA showed that F= 1.258, P=0.235 〉0.05. [Conclusion]Although A2/O technology can decrease environmental estrogens （EEs） to a certain extent, it is not statistically significant. Hence we conclude that this tech- nology does not implement significant effect on reducing or eliminating EEs.

关 键 词：高效液相色谱一串联质谱法 双酚A A2/O工艺 

分 类 号：O658[理学—分析化学]