基于肺与大肠相表里研究电针对大承气汤在急性胰腺炎大鼠体内药动学的影响  

Research on the Effect of Electro-acupuncture on Pharmacokinetics of Da-Cheng-Qi Decoction on Rats with Severe Acute Pancreatitis Based on the Interior-exteriorly Related to the Lung and Large Intestine

作　　者：薛桂君[1] 黄伟[2] 李娟[1] 郭徽[1] 赵先林[1] 唐文富[1] 陈光远[1] 

机构地区：[1]四川大学华西医院,四川成都610041 [2]成都市第五人民医院,四川成都611130

出　　处：《针灸临床杂志》2015年第10期77-81,97,共6页Journal of Clinical Acupuncture and Moxibustion

摘　　要：目的：研究电针肺与大肠背俞穴对中药复方大承气汤在重症急性胰腺炎（SAP）大鼠体内的药动学影响,为阐明针药合用机理奠定药动学基础。方法：将12只雄性SD大鼠随机分为针刺组和中药组,每组各6只,采用3%牛黄胆酸钠逆行胰胆管注射构建SAP大鼠模型。针刺组造模后予以电针＂肺俞＂、＂大肠俞＂处理,每日两次,每次20 min,连续2天。两组大鼠均于造模后24 h灌胃中药大承气汤,并分别于灌胃后10 min、20 min、40 min以及1 h、2 h、3 h、4 h、8 h、12 h、24 h尾静脉取血。利用液质联用技术测定各个时间点大承气汤主要成分血药浓度,并拟合计算其药动学参数。结果：针刺组芦荟大黄素Cmax明显低于中药组芦荟大黄素Cmax,两组比较有统计学差异（P〈0.05）;中药组大黄酸及芦荟大黄素AUC（0-24 h）均大于针刺组,两组比较有统计学差异（P〈0.05）。针刺组大承气汤复方中大黄素、大黄酸、芦荟大黄素、大黄酚、大黄素甲醚、柚皮苷和厚朴酚Tmax达峰时间均比中药组提前,大黄素、大黄酚、大黄素甲醚、柚皮苷、橙皮苷、柚皮素、柚皮苷、厚朴酚Cmax均大于中药组,但两组比较均无统计学差异。结论：电针＂肺俞＂、＂大肠俞＂可以影响大承气汤在SAP大鼠体内药动学过程。Objective： This experiment researched the effect of electro- acupuncture on pharmacokinetcs of Da- Cheng- Qi Decoction（ DCQD） in rats with severe acute pancreatitis（ SAP） based on the theory of interior- exterior relationship of lung and large intestine and the combination of Back- shu and Front- mu points treating both visceral and bowels＇ diseases. To lay a foundation on the pharmacokinetics for illustrating the mutual impacts of acupuncture and traditional Chinese medicine. Methods： Twelve Sprague- Dawley rats were divided into an acu- group and a control group randomly（ n = 6）. SAP rat models were established by injecting 3%sodium taurocholate into biliopancreatic duct through duodenal wall of both groups and manipulated electro- acupuncture on both BL13 and BL25 twice a day,20 minutes each time and lasted for 2 days. All rats were given DCQD by gavage 24 hours after the SAP model induced and blood samples were taken through their caudal veins on the time points of 10,20,40 minutes and 1,2,3,4,8,12,24 hours after the gavage. The plasma concentrations of each DCQD main components were measured and the parameters of pharmacokinetics were calculated by Liquid Chromatography- Mass Spectrometry / Mass Spectrometry. Results： The Cmax and AUC of aloe- emodin as well as the AUC of rhein were obviously lower in acu- group than those in control group（ P〈0. 05） while the Cmax and AUC of other DCQD components were higher than those in control group with no statistical differences. The Tmax of emodin,rhein,rheochrysidin,chrysophanol,aloe- emodin,naringin and magnolol as well as the T1 /2 of emodin,rhein,rheochrysidin,chrysophanol,hesperidin,naringin,naringenin and magnolol were advanced in acu- group than those in control group with no statistical differences. Conclusion： Electro- acupuncture can affect the pharmacokinetics of some components from DCQD in SAP rats.

关 键 词：重症急性胰腺炎 针药结合 电针 肺与大肠相表里 俞募配穴法 药动学 

分 类 号：R246.1[医药卫生—针灸推拿学]