葡萄糖6磷酸异构酶和烯醇化酶1可预测人类精子冷冻复苏后活力  

Glucose-6-phosphate isomerase(GPI) and Enolasel(ENO1)predict the motility of cryopreserved spermatozoa

作　　者：蒋旭平[1,2] 王尚乾[1] 王巍[1] 徐杨[1] 汤井源 徐震[1] 周伟民[2] 张炜[1] 

机构地区：[1]南京医科大学第一附属医院泌尿外科,南京210029 [2]宜兴市人民医院泌尿外科

出　　处：《中国男科学杂志》2015年第8期27-32,共6页Chinese Journal of Andrology

基　　金：国家自然科学基金面上项目(81370781)

摘　　要：目的 比较葡萄糖6磷酸异构酶（GPI）和烯醇化酶1（ENO1）在人类精子中不同耐冻能力组之间表达的差异,寻找预测人类精子冻融后活力可能的标志物.方法 收集42例合格供精志愿者的新鲜精液样品,依据冻融后精子活力的不同,将符合条件的样品分为耐冻组和不耐冻组,应用蛋白质印迹（Western blot）技术比较两组样品GPI和ENO1蛋白在表达量上的差异,利用免疫荧光技术验证Western blot结果并观察两种蛋白在精子中的分布.结果 在两组样品之间,GPI和ENO1都表现出明显的差异.GPI和ENO1在耐冻组的表达量均高于不耐冻组（分别为P＜0.01,P＜0.05）,而蛋白在精子中的分布未见明显组间差异.结论 GPI和ENO1可以作为标志物在冻存前预测人类精子冷冻复苏后的活力.Objective To explore the probable biomarker of human sperm to predict the motility of cryopreserved spermatozoa. Methods 42 semen samples from 42 donors satisfied the minimal values of sperm quality before eryopreservation and were divided into good freezability ejaculates and poor freezability ejaculates according to progressive motility of the sperm after thawing. The level of glucose-6-phosphate isomerase （GPI） and enolasel （ENO1） in semen samples from each group before cryopreservation were comparatively analyzed by western blot. Immunofluorescence staining was also performed for validation and distribution of GPI and ENO 1. Results Levels of GPI （P〈0.01） and ENO 1 （P〈0.05） in semen samples were both significantly higher in GFE than that in PFE. There was no difference in location of the two proteins in sperm between two groups. Conclusion GPI and ENO1 can be used as markers to predict sperm freezabilitv before starting, the ervooreservation nrocedure.

关 键 词：精液保存 葡糖-6-磷酸异构酶 磷酸丙酮酸水合酶 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]