根际优势细菌对甜樱桃幼树光合及根系活力的影响  

Effects of Plant Growth-promoting Rhizobacteria on Photosynthesis and Root Vitality of Sweet Cherry Saplings

作　　者：周文杰[1] 吕德国[1] 杨丹丹[1] 秦嗣军[1] 

机构地区：[1]沈阳农业大学园艺学院,辽宁省果树品质发育与调控重点实验室,沈阳110866

出　　处：《吉林农业大学学报》2015年第5期555-561,567,共8页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(30871688,30900967);高等学校博士学科点专项科研基金项目(20122103110012);辽宁省高等学校果树栽培与生理生态创新团队项目(LT2014014);辽宁省高等学校优秀人才支持计划项目(LJQ2014070)

摘　　要：通过接种7种甜樱桃根际优势促生细菌,检测植株光合及荧光参数、根系活力和根系呼吸等指标变化,探讨根际优势促生菌对植株生理代谢的影响。结果表明:绿针假单胞菌(Pse1)、申氏不动杆菌(Aci1)、争论贪噬菌(Var)、假单胞菌(Pse2)及边缘假单胞菌(Pse3)菌株均显著提高净光合速率(Pn)和水分利用率(WUE);鲁氏不动杆菌(Aci2)和雷氏普罗威登斯菌(Pro)处理的叶片胞间CO2浓度(Ci)显著低于对照;PSⅡ最大光化学效率(Fv/Fm)和PSⅡ的潜在活性(Fv/Fo)均无显著变化;Pse1、Var、Pse2及Pse3显著提高根系活力,其中Pse3菌株接种后根系活力最高,达对照的2.0倍;Aci1处理根系总呼吸速率上升,Pse1、Pse2、Pse3和Pro处理影响了根系电子传递途径的分配比例,但各菌株处理对根系呼吸基础生化途径比例无显著影响。接种根际优势促生细菌整体上提高了樱桃植株净光合速率、水分利用效率和根系活力,不同菌属菌株处理效应存在差异。In this study,effects of plant growth-promoting rhizobacteria（ PGPR） on plant physiological metabolism,photosynthetic and fluorescence parameters,root vitality and root respiration were detected by inoculation of 7 types of plant growth-promoting rhizobacteria of sweet cherry. The results showed that net photosynthetic rate（ Pn） and water use efficiency（ WUE） were significantly increased in Pseudomonas chlororaphis（ Pse1）,Acinetobacter schindleri（ Aci1）,Variovorax paradoxus（ Var）,Pseudomonas sp.（ Pse2） and Pseudomonas marginalis（ Pse3） treatments,whereas the stomatal intercellular CO2concentration（ Ci） in Acinetobacter lwoffii（ Aci2） and Providencia rettgeri（ Pro） treatments were significantly lower than those in the control. Maximum photochemical efficiency of PSⅡ（ Fv / Fm） and potential activity of PSⅡ（ Fv / Fo） changed slightly in the controland 7 treatments. Root vitalities were significantly increased in Pse1,Var,Pse2 and Pse3,especially in Pse3,which was 2. 0 times higher than that in the control. Root total respiration rate was increased significantly in Aci1 treatment. Contribution of root electron transport pathway was altered in Pse1,Pse2,Pse3 and Pro treatments,whereas the basis biochemical pathways were changed slightly in all the treatments and the control. In conclusion,PGPR inoculation increases net photosynthetic rate,water use efficiency and root vitality,but treatment effects are different among bacterial strains in different species.

关 键 词：樱桃 促生细菌 光合参数 根系 呼吸代谢 

分 类 号：S662.5[农业科学—果树学]