鲜无蹼壁虎抗肿瘤活性成分提取分离的初步研究  

Preliminary Study on Extraction and Separation of Anti-tumor Ingredients from Fresh Gekko Swinhonis

作　　者：李红霞[1] 康建功[2] 张仕状[2] 

机构地区：[1]解放军第89医院,山东潍坊261031 [2]潍坊医学院附属医院

出　　处：《中国药师》2015年第11期1841-1843,共3页China Pharmacist

基　　金：国家自然科学基金项目(编号:30572323)

摘　　要：目的:研究鲜无蹼壁虎抗肿瘤活性成分的提取分离。方法:不同浓度乙醇以不同方法进行提取,提取物经过反复冻融脱蛋白质脱脂肪分离后经高效液相法分离纯化,采用MTT比色法检测其抗肿瘤活性。结果:85%乙醇超声提取工艺Ⅲ组A值为0.548±0.045,抑瘤率最高,与空白对照组比较,差异有统计学意义(P<0.05)。结论:药效学实验结果表明85%乙醇超声提取为最佳提取工艺。Objective：To study the extraction and separation of anti-tumor active ingredients from fresh Gekko swinhonis. Meth-ods：The extraction was carried out with ethanol at different concentrations by various methods. The extract was purified by HPLC after deproteinization and degreasing using a repeated freezing and thawing method. MTT was used to detect the antitumor activity of the ex-tract. Results：The A value of extraction process groupⅢwith 85% ethanol ultrasonic extraction was （0. 548 ± 0. 045） with the high-est tumor inhibition rate, and compared with the control group, the difference was significant （P〈0. 05）. Conclusion：The results of pharmacodynamics show that the optimum extraction technology is ultrasonic extraction with 85% ethanol.

关 键 词：鲜无蹼壁虎 抗肿瘤活性成分 提取分离 

分 类 号：R284.2[医药卫生—中药学]