HPLC法测定盐酸左氧氟沙星眼用即型凝胶的含量  

Determination of Levofloxacin Hydrochloride Ophthalmic in situ Gel by HPLC

作　　者：刘彩霞 杨琪 董爱梅 邢玉仁 

机构地区：[1]山东省药学科学院山东省生物药物重点实验室山东福瑞达医药集团公司山东省黏膜与皮肤给药技术重点实验室,济南250101

出　　处：《中国药师》2015年第11期1987-1989,共3页China Pharmacist

基　　金：山东省科技攻关计划(编号:2006GG2202062)

摘　　要：目的：建立测定盐酸左氧氟沙星眼用即型凝胶中左氧氟沙星含量的HPLC方法。方法：采用Zorbax C18（150 mm x4.6 mm,5μm）色谱柱,以己烷磺酸钠溶液-甲醇（72∶28）为流动相,流速为1.0 ml·min-1；检测波长为293 nm,柱温为40℃；进样量为10μl。结果：左氧氟沙星在0.0403-0.4030 mg·ml-1范围内线性关系良好（r=1.0000）,平均回收率为99.8%（RSD=1.08%,n=9）。结论：该方法准确、简便、快速,适用于测定盐酸左氧氟沙星眼用即型凝胶中左氧氟沙星的含量。Objective：To establish an HPLC method for the determination of levofloxacin in levofloxacin hydrochloride ophthalmic in situ gel. Methods：The chromatographic column was Agilent Zorbax C18（150 mm x4.6 mm,5μm）. The mobile phase consisted of hexane sulfonic acid sodium solution-methanol（72∶28）. The flow rate was 1. 0 ml·min-1. The detection wavelength was 293 nm. The chromatographic column temperature was 40℃. The injection volume was 10 μl. Results：The calibration curve was linear within the range of 0. 040 3-0. 403 0 mg·ml-1（r=1. 000 0） for levofloxacin. The average recovery was 99. 8% （RSD=1. 08%,n=9）. Conclusion：The method is accurate, simple and rapid, and suitable for the content determination of levofloxacin in levofloxacin hydro-chloride ophthalmic in situ gel.

关 键 词：盐酸左氧氟沙星眼用即型凝胶 高效液相色谱法 左氧氟沙星 

分 类 号：R927.2[医药卫生—药学]