同步辐射X线相衬成像技术在兔髌骨-髌腱连接点纤维软骨细胞的三维可视化应用研究  

Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography

作　　者：陈华斌[1] 胡建中[2] 周京泳[1] 郑成[1,3] 王占稳 陈灿[1] 曹勇[2] 殷宪振[4] 吕红斌[1] 

机构地区：[1]中南大学湘雅医院运动医学科中南大学运动医学研究中心,长沙410008 [2]中南大学湘雅医院脊柱外科,长沙410008 [3]武汉体育学院医院,武汉430079 [4]中国科学院上海药物研究所药物释放系统研究中心,上海201203

出　　处：《核技术》2015年第11期1-8,共8页Nuclear Techniques

基　　金：国家自然科学基金(No.8117169;No.84172072)资助

摘　　要：利用同步辐射X射线相衬成像技术(Synchrotron Radiation-based X-ray Phase-contrast Computed Tomography,SR-XPCT)对兔髌骨-髌腱连接点(Patella-Patellar Tendon Junction,PPTJ)移行区的纤维软骨细胞进行三维可视化及量化表征。正常骨成熟的新西兰PPTJ经多聚甲醛固定、梯度酒精脱水后,于上海光源X射线成像与生物医学应用光束线站进行扫描;采集的原始图像经数字减影、相位恢复、切片重构、位数转换后进行三维重构分析;标本扫描后进行传统组织学验证。SR-XPCT能成功获取PPTJ及其移行区纤维软骨细胞的三维高清图像,并对移行区纤维软骨细胞进行了量化表征:平均直径为(10.139±1.265)?m;平均体积为(291.187±87.283)μm3,体积分布在200-400μm3之间的占75.4%;平均球形度为0.711±0.079,球形度在0.605-0.805之间的纤维软骨细胞约占84.9%。SR-XPCT作为一种先进的、直观准确的可视化骨腱连接点的三维微观形态结构研究方法,为骨腱连接点损伤修复的三维形态学研究提供了新方法、新思路。Background： Synchrotron radiation-based X-ray phase-contrast computed tomography （SR-XPCT） is an advanced tool for nondestructive analysis of three dimensional inner microstructure of samples, and plays an important part in the researches such as spinal cord microvasculature and lung bronchium.Purpose： This study was to investigate the three-dimensional morphology of fibrochondrocytes in rabbit patella-patellar tendon junction （PPTJ） using SR-XPCT, and develop an advanced imaging method.Methods：Three PPTJs were harvested from healthy mature female New Zealand rabbits. The specimens were scanned by SR-XPCT at BL13W1 of Shanghai Synchrotron Radiation Facility （SSRF） in China. The tomographic image was captured by Charge Coupled Device （CCD） detector with a 0.65-μm resolution. The three-dimensional visualization images of PPTJ were reconstructed by VG Studio Max. After that, the specimens were embedded by paraffin for Safranin O/Fast-green staining.Results：High-resolution three-dimensional visualization images of PPTJ and the parameters of the chondrocytes in fibrocartilage zone were successfully obtainedvia the SR-XPCT. With spherical and ellipsoidal morphologic features and its configuration characteristics in three dimension, the fibrochondrocytes revealed by SR-XPCT were consistent with the results of traditional histological staining. The parameters of chondrocytes showed that the mean diameter of chondrocyte was （10.139±1.265）μm, the mean volume was （291.187±87.283）μm3 and 75.4% of the chondrocytes’ volume ranged from 200μm3 to 400μm3, the mean sphericity degree was 0.711±0.079, and 84.9% of chondrocytes’ sphericity degree ranged from 0.605 to 0.805.Conclusion：The three-dimensional microstructure of the PPTJ was intuitively, accurately and undistortedly realized by the SR-XPCT, which may act as a novel instrument to access the three-dimensional microstructure changes during the PPTJ healing process.

关 键 词：同步辐射 相位衬度成像 骨腱连接点 纤维软骨细胞 三维 

分 类 号：TL99[核科学技术—核技术及应用]