HPLC法同时测定山栀茶中的3种苷类成分  

Simultaneous determination of three glycosides in Pittosporum glabratum by HPLC

作　　者：乔里[1,2] 汪石丽 关焕玉[3] 廖尚高[3] 刘丽娜[1] 李勇军[3] 

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵州医科大学附属医院,贵州贵阳550004 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004

出　　处：《中成药》2015年第11期2448-2451,共4页Chinese Traditional Patent Medicine

基　　金：国家科技支撑计划课题(2013BAI11B01);贵州省科学技术基金项目(20102217);贵州省中药现代化科技产业研究开发专项(黔科合中药字[2013]5062;黔科合重G字[2013]4001);贵州医学院院基金(2012021号)

摘　　要：目的建立HPLC法同时测定山栀茶Pittosporum glabratum Lindl.中3,4,5-三甲氧基苯-1-O-β-D-呋喃芹糖-（1→6）-β-D-吡喃葡萄糖苷、丁香脂素-4,4＇-O-双-β-D-葡萄糖苷、异落叶松树脂醇-9＇-O-β-D-葡萄糖苷的含有量。方法山栀茶提取液分析采用Diamosil C18色谱柱（4.6 mm×250 mm,5μm）;乙腈-0.1%磷酸水溶液为流动相,梯度洗脱;体积流量1.0 m L/min;柱温30℃;检测波长210 nm。结果这3种成分均达到基线分离,线性关系良好（r≥0.999 9）,加样回收率在98.5%~100.5%之间,RSD均小于3%。结论该方法灵敏准确、可靠稳定,可用于山栀茶药材的质量评价。AIM To establish an HPLC method for simultaneously determining the contents of 3,4,5-trimethoxyphenol-1-O-β-D-apiofuranosyl-（ 1→6）-β-D-glucopyranoside,syringaresinol-4,4＇-bis-O-β-D-glucopyranoside and isolariciresinol 9＇-O-β-D-glucopyranoside in Pittosporum glabratum Lindl. METHODS The analysis of P. glabratum extract was performed on Diamonsil C18column（ 4. 6 mm × 250 mm,5 μm） with gradient elution,mobile phase was acetonitrile-0. 1% phosphoric acid aqueous solution,flow rate was 1. 0 m L / min,detection wavelength was set at 210 nm,and column temperature was maintained at 30 ℃. RESULTS These three constituents reached the baseline separations with good linear relationships（ r≥0. 999 9）,whose average recoveries were in the ranges of 98. 5%- 100. 5%,and RSDs were all less than 3%. CONCLUSION This method is sensible,accurate,reliable and stable,which can be used for the quality evaluation of P. glabratum.

关 键 词：山栀茶 3 4 5-三甲氧基苯-1-O-β-D-呋喃芹糖-(1→6)-β-D-吡喃葡萄糖苷 丁香脂素-4 4'-O-双-β-D-葡萄糖苷 异落叶松树脂醇-9'-O-β-D-葡萄糖苷 HPLC 

分 类 号：R284.1[医药卫生—中药学]