骨性关节炎患者膝关节软骨及软骨下骨中miR-214的表达  

Expression level of mi R-214 in cartilage and subchondral bone of patients with osteoarthritis

作　　者：余晓明[1] 袁雪凌[1] 孟昊业[1] 孙振[1] 尹合勇 郭全义[1] 彭江[1] 汪爱媛[1] 卢世璧[1] 

机构地区：[1]解放军总医院骨科研究所,北京100853 [2]南开大学,天津300071

出　　处：《解放军医学院学报》2015年第11期1131-1133,共3页Academic Journal of Chinese PLA Medical School

基　　金：国家"863"计划项目(2012AA020502);全军十二五重点项目(BWS11J025);国家"973"重点基础研究发展规划项目(2012CB518106)~~

摘　　要：目的探讨不同Kellgren-Lawrence X线分级的骨性关节炎患者膝关节软骨及软骨下骨中Micro RNA-214(mi R-214)的表达,为进一步研究mi R-214在骨性关节炎发生和发展中的作用提供依据。方法收集截肢患者及骨性关节炎全膝关节置换患者膝关节胫骨平台40例,根据K-L评分标准将其分为Ⅰ级组(10例)、Ⅱ级组(10例)、Ⅲ级组(10例)、Ⅳ级组(10例)。将样本进行软骨及软骨下骨分离后,运用实时定量PCR检测不同分组中软骨及软骨下骨中mi R-214的表达情况。结果在所有软骨及软骨下骨中均能检测到mi R-214的表达。比较4组之间mi R-214的表达情况,发现4组软骨中mi R-214的表达有明显差异(P<0.05),并且呈现逐渐升高的趋势;骨性关节炎发展的严重程度与mi R-214的表达水平密切相关。Ⅲ级组中软骨下骨中的mi R-214的表达量明显高于其他3组(P<0.05)。结论 mi R-214的表达水平与骨性关节炎发展的严重程度密切相关,表明mi R-214可能与骨性关节炎的发生、发展有密切联系。Objective To detect the expression level of micro RNA-214（mi R-214） in cartilage and subchondral bone of different graded osteoarthritis（OA）, which was classified by X-ray diagnosis criteria of Kellgren and Lawrence, and provide theoretical foundation for researching the role of mi R-214 in OA development. Methods Tibial plateaus of OA patients were collected from those who underwent total knee arthroplasty（TKA） and amputation. Forty samples were divided into 4 groups by K-L osteoarthritis scoring system： gradeⅠgroup（n=10）, gradeⅡgroup（n=10）, gradeⅢgroup（n=10）, and gradeⅣgroup（n=10）. After getting the samples, we segregated cartilage and subchondral bone, and the expression level of mi R-214 by real-time PCR was tested. Resultsmi R-214 was examined in all cartilage and subchondral bone. The expression level of mi R-214 was compared in four groups, and it showed a rising trend with significant difference（P〈0.05）. Positive correlation was found between the severity of OA and the expression level of mi R-214. Among the four groups, the level of mi R-214 in subchondral bone was highest. Conclusion The expression level of mi R-214 in 4 groups is keeping rising, which is related to the severity of OA progression. And the expression level of mi R-214 also has obvious change in subchondral bone. Our findings indicate that mi R-214 has intertwined relationshiP〈0with the development and progression of OA, which provides theoretical foundation for OA development and treatment.

关 键 词：骨性关节炎 软骨 软骨下骨 microRNA 

分 类 号：R684.3[医药卫生—骨科学]