苜蓿SSR遗传距离与杂种优势的相关性分析  

Correlation Analysis of SSR Genetic Distance and Heterosis on Alfalfa

作　　者：乌云塔娜[1] 石凤翎[1] 薛晓兰 石凤玲[1] 崔楠[1] 

机构地区：[1]内蒙古农业大学生态环境学院,呼和浩特010019

出　　处：《植物遗传资源学报》2015年第6期1237-1244,共8页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31260576)

摘　　要：利用SSR标记技术对40份苜蓿材料（6个雄性不育株系和34个苜蓿品种）的遗传距离进行分析,并利用其中的6个不育株系与14个苜蓿品种测交,进一步对遗传距离（GD）、产量配合力与杂种优势效应进行相关性分析。结果表明,25对SSR引物共扩增出189条谱带,其中多态性条带136条,平均多态性位点百分率为69.23%;40份苜蓿材料的遗传距离为0.1818~0.9091,平均0.4544;各亲本一般配合力及遗传距离均与杂种优势效应存在显著正相关,其中亲本一般配合力与杂种优势的相关性高于遗传距离。因此,仅以SSR遗传距离还不足以准确地组配强优势组合,需结合各性状配合力的分析,以充分发挥杂种优势效应。Genetic distances of 40 alfalfa materials were analyzed using SSR markers. 6 sterility strain and （six ma/e sterile lines and 34 alfalfa breeding materials） 14 alfalfa varieties were used to find the difference in the coupling relationships among genetic distance（ GD）, yield combining ability and heterosis effect through test cross. The results showed that 25 SSR primers pairs amplified 189 bands,including 136 polymorphic bands with the average polymorphism loci percentage of 69.23 %. The mean value of genetic distance was 0. 4544 among the different alfalfa materials ,and varied from 0. 1818 to 0. 9091. Both general combining ability and GD had significantly positive relationship with alfalfa heterosis. The correlation between general combining ability and alfalfa heterosis was higher than GD and heterosis. To improve heterosis effect, therefore, SSR genetic distance was not enough to assemble strong hetrosis combination. Furthermore, estimating all of characters combining ability would be needed.

关 键 词：苜蓿雄性不育系 SSR标记 牧草产量配合力 遗传距离 杂种优势 

分 类 号：S541.9[农业科学—作物学]