脓毒症中血小板活化及造血系细胞特异性蛋白-1磷酸化的相关性研究  

The study of relevance between platelet activity and HS1 phosphorylation in sepsis

作　　者：许磊[1] 郭东风[1] 刘国荣[2] 施琴[1] 赵昌明[1] 杭敏[1] 

机构地区：[1]上海市浦东新区公利医院急诊科,上海200135 [2]宁夏医科大学研究生院

出　　处：《中华急诊医学杂志》2015年第11期1253-1256,共4页Chinese Journal of Emergency Medicine

基　　金：上海市浦东新区科技发展基金创新资金（PKJ2012.Y55）

摘　　要：目的 探讨脓毒症时血小板功能的变化以及血小板中造血系细胞特异性蛋白-1（HS1）浓度和磷酸化HS1的变化和参与调节HS1磷酸化的因子.方法 收集并分离150例脓毒血症患者血小板和50例健康人富血小板血浆,使用微孔板吸附法和血小板凝集仪对两组人群血小板的黏附功能进行比较,同时使用ATP检测试剂盒检测两组人群洗涤血小板中ATP含量,对其释放功能进行比较;然后通过免疫印迹法对两组人群血小板总HS1 （t-HS1）和磷酸化HS1 （p-HS1）含量进行比较,随后使用LPS刺激分离健康人组血小板,并使用Src、Syk激酶特异性抑制剂验证该因子对HS1活化的调节作用.结果 数据显示脓毒症组血小板计数、血小板分布宽度（platelet distribution width,PDW）、平均血小板体积（mean platelet volumn,MPV）较健康人组差异具有统计学意义（P＜0.01）;脓毒症患者血小板黏附能力、聚集能力和释放能力均显著强于健康人;脓毒症血小板HS1以及p-HS1均显著高于健康人组,使用Src、Syk激酶抑制剂PP2和piceatannol可以显著的抑制LPS诱导的血小板HS1的磷酸化.结论 脓毒症中血小板HS1蛋白的与血小板功能密切相关,且有望成为脓毒症治疗的靶点.Objective To explore the change of function and expression of hematopoietic lineage cell specific protein-1 （HS1） and phosphorylated HS1 （p-HIS） and factors devoting to HS1 phosphorylation in platelet with sepsis.Methods Plasma with rich platelet was collected from 150 sepsis patients and 50 healthy subjects, and comparison of platelets adhesion and aggregation were detected by micro-pore method and platelet aggregation instrument.Meanwhile the ATP concentrations of washed platelet of two groups were detected by the kit to compare release reaction.And then total HS1 （t-HIS） and p-HS1 of platelet from two groups were compared by using western blot.Afterwards the specific inhibitors of Src and Syk were used to verify the HS1 activation regulated by Src and Syk in LPS-induced cell model.Results The significant differences were present between healthy subjects and sepsis patients in platelet counts, platelet distribution width （PDW） and mean platelet volume （MPV） （P 〈 0.01）.The data showed the sepsis patients had greater ability than healthy subjects in adhesion, aggregation and release reaction.Meanwhile the platelets of sepsis patients had higher concentration of t-HS1 and p-HS1 than healthy subjects, and the specific inhibitors of Src and Syk , PP2 and piceatannol, inhibited the increase in p-HS1 in LPS-induced cell model.Conclusions Function of platelet is closely related to HS1 in sepsis and it will be a target for sepsis therapy.

关 键 词：脓毒症 血小板 造血系细胞特异蛋白-1 活化 

分 类 号：R459.7[医药卫生—急诊医学]