丹龙醒脑方对脑缺血再灌注大鼠神经功能和脑梗死面积的影响  

Effects of Danlong Xingnao prescription on neurological function and infarct area in rats with cerebral ischemia-reperfusion

作　　者：梁昊[1] 徐瑾瑜 张秋雁[1] 唐金强[1] 朱伟[1] 苏剑峰[1] 唐映红[1] 逯晶[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]华恩堂养生管理有限公司,湖南长沙410000

出　　处：《湖南中医杂志》2015年第10期147-149,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省高校创新平台开放基金项目(编号:12K090)

摘　　要：目的:研究丹龙醒脑方对脑缺血再灌注大鼠神经功能和脑梗死面积的影响。方法:用线栓法制备大鼠大脑中动脉栓塞缺血再灌注模型。造模成功后将48只SD雄性大鼠随机分为模型组,假手术组,尼莫地平组,丹龙醒脑方大、中、小剂量组,每组各8只。分别灌胃等体积蒸溜水、尼莫地平片与不同剂量的丹龙醒脑方。连续灌胃7d后观察神经功能症状评分与脑梗死面积。结果:神经功能评分、脑梗死面积方面,模型组,尼莫地平组及丹龙醒脑方大、中、小剂量组与假手术组比较,差异均有统计学意义(P<0.01);尼莫地平组及丹龙醒脑方大中小剂量组与模型组比较,差异均有统计学意义(P<0.05);丹龙醒脑方各剂量组组间比较,差异均无统计学意义(P>0.05)。丹龙醒脑方大中小剂量组神经功能评分与尼莫地平组比较,差异均无统计学意义(P>0.05);丹龙醒脑方大中剂量组与尼莫地平组比较,差异均有统计学意义(P<0.05)。结论:丹龙醒脑方能改善脑缺血再灌注大鼠神经症状,减少脑缺血再灌注脑梗死面积。Objective： To investigate the effects of Danlong Xingnao prescription on neurological function and infarct area in rats with cerebral ischemia- reperfusion. Methods： Forty- eight male Sprague- Dawley rats were randomly and equally divided into model group,sham operation group,nimodipine group,and high-,medium-,and low- dose Danlong Xingnao prescription groups. All rats except those in the sham operation group were treated with intraluminal suture occlusion of the middle cerebral artery to produce the animal model of ischemia- reperfusion. Drugs were administrated by gavage after successful model establishment. Rats in the sham operation group were given an equal volume of distilled water by gavage for 7 continuous days. The score of neurological function and infarct area were compared between groups,and morphological changes in rat hippocampal cells were observed. Results：Compared with the sham operation group,all other groups had significantly higher scores of neurological function and significantly larger infarct areas（ P 〈0. 01）. Compared with the model group,the nimodipine group and high-,medium-,and low- dose Danlong Xingnao prescription groups had significantly improved scores of neurological function and infarct areas（ P〈 0. 05）. The high- and medium- dose Danlong Xingnao prescription group had the most significant improvement in infarct area. However,there was no significant difference in improvement in infarct area between the nimodipine group and the high- or medium- dose Danlong Xingnao prescription group（ P 〉0. 05）. The observation of cell morphology demonstrated that more cells survived in the high-,medium-,and low- dose Danlong Xingnao prescription groups than in the model group. Conclusion： Danlong Xingnao prescription can improve neurological function,reduce infarct area,and prevent hippocampal neuron death in rats with cerebral ischemia- reperfusion.

关 键 词：脑缺血再灌注 丹龙醒脑方 神经功能评分 脑梗死面积 实验动物 

分 类 号：R285.5[医药卫生—中药学]