加德纳菌感染流行病学调查及实验室快速检测方法评价  

Epidemiological investigation of Garderella and evaluation of rapid laboratory detection methods

作　　者：黄伟忠[1] 张莉[1] 张扬[1] 唐景云[1] 张鲍虎[1] 

机构地区：[1]深圳市坪山新区人民医院检验科,广东深圳518118

出　　处：《中国热带医学》2015年第10期1198-1200,1216,共4页China Tropical Medicine

基　　金：深圳市坪山新区医疗卫生科研配套资助项目(No.201325)

摘　　要：目的分析加德纳菌感染流行病学特征,评价免疫荧光法、唾液酸酶法的诊断效能。方法取680例阴道分泌物标本,采用免疫荧光法检测加德纳菌,同时进行唾液酸酶测定,以Amsel法为＂金标准＂,比较两种方法的诊断结果。结果 680例受检妇女中,检出GV阳性285例,20~30岁年龄组阳性率明显高于31~40、41~50、〉50岁年龄组（P〈0.05、0.01）;31~40岁年龄组明显高于〉50年龄组（P〈0.01）;不孕不育、不良怀孕、细菌性阴道病患者阳性检出率分别为16.67%、23.30%、55.87%;免疫荧光法灵敏度、特异度、准确率、阳性预测值、阴性预测值均略高于唾液酸酶法,差异无统计学意义（95.44%vs 89.47%,91.90%vs 90.63%,93.38%vs 91.90%,89.47%vs 87.33%,96.54%vs 92.27%）（P〉0.05）。结论细菌性阴道病与加德纳菌感染有明显的年龄相关性,免疫荧光法具有灵敏度高、特异性强的特点。Objective To analyze the epidemiological characteristics of Gardnerella vaginalis（GV）and to evaluate thediagnosis efficiency of immunofluorescence assay and sialidase method. Methods A total of 680 vaginal secretion specimenswere collected to detected the gardnerella vaginalis using immunofluorescence assay method and golden standard＂Amsel＂method and the detectiong results of the two methods were compared. Results Among the 680 detected women, 285 werepositive for GV, positive rate in women of 20-30 years old was significantly higher than that in 31-40, 41-50 and over 50 years old（P〈0.05, 0.01）; positive rate in 31- 40 year old group were significantly higher than that in over 50 years old group（P〈0.0）;Positive rates in women with infertility, adverse pregnancy, bacterial vaginal disease were 16.67%, 23.30% and55.87%,respectively; The sensitivity, specificity,accuracy, positive predictive value, negative predictive value ofimmunofluorescence were slightly higher than those of the sialidase without statistical significance（95.44% vs 89.47%,91.90% vs 90.63%, 93.38% vs 91.90%, 89.47% vs 87.33%, 96.54% vs 92.27%）（P〉0.05）. Conclusion Bacterial vaginosisand GV infection present obvious association with ages, the immunofluorescence assay method is characterized by its highsensitivity and specificity.

关 键 词：阴道加德纳菌 免疫荧光法 唾液酸酶法 诊断效能 

分 类 号：R711.3[医药卫生—妇产科学]