白藜芦醇对妊娠期糖尿病大鼠脂代谢紊乱调控的实验研究  

Experimental study of resveratrol regulating lipid metabolism disorder of rats with gestational diabetes

作　　者：邢宝恒[1] 曹亚磊[1] 董秀娟[1] 李芹[1] 

机构地区：[1]河北省沧州市中心医院产二科,061001

出　　处：《中国计划生育学杂志》2015年第11期733-738,共6页Chinese Journal of Family Planning

摘　　要：目的:研究白藜芦醇(Resveratrol,Res)对妊娠期糖尿病大鼠脂代谢紊乱的调控作用。方法:取60只妊娠5d SD大鼠,通过一次性腹腔注射链脲佐菌素(STZ)35 mg/kg制备妊娠期糖尿病大鼠模型,随机分为5组:妊娠糖尿病模型对照组,白藜芦醇(60、120和240 mg/kg)治疗组和盐酸二甲双胍(200 mg/kg)阳性对照组,每组12只;另取12只妊娠5dSD大鼠作为正常妊娠对照组,12只非妊娠雌性大鼠作为正常非妊娠对照组。分别于给药前和给药治疗第7、14d检测空腹血糖、胰岛素水平;治疗满2周后,测量各组大鼠体重,测定总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)含量;测定血浆中脂肪因子指标:瘦素(Leptin)、脂联素(Adiponectin)、趋化素(Chemerin)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平。结果:与正常妊娠对照组相比,妊娠糖尿病模型对照组大鼠胰岛素水平显著降低、空腹血糖水平显著升高,体重显著升高,血清中TC、TG、LDL-C含量显著升高,HDLC含量显著降低,Leptin、Chemerin、TNF-α、IL-6水平显著升高,Adiponectin水平显著降低。与妊娠期糖尿病模型组相比,白藜芦醇治疗组大鼠血清中TC、TG含量显著降低;胰岛素水平显著升高,血糖、体重、血清中LDL-C含量显著降低,血浆中Leptin、Chemerin、TNF-α、IL-6水平显著降低,血浆中Adiponectin显著升高,且呈剂量依赖性关系。与盐酸二甲双胍治疗组相比,白藜芦醇240mg/kg治疗组大鼠胰岛素水平显著升高,血清中TC、TG、LDL-C含量显著降低,血浆中Leptin、Chemerin、TNF-α、IL-6水平显著降低且Adiponectin水平显著升高,差异均具有统计学意义(P<0.01)。结论:白藜芦醇对妊娠期糖尿病大鼠具有剂量依赖性的提高胰岛素分泌、降低血糖、降低体重、调节血脂和血浆脂肪因子的作用,并且治疗量(240mg/kg)的作用强于盐酸二甲双胍。Objective： To investigate the effects of resveratrol regulating lipid metabolism disorder of rats with gesta- tional diabetes. Methods： 60 rats with gestation for five days were intraperitoneal injected streptozotocin（STZ） 35mg/ kg to make gestational diabetic model. All of the rats were randomly divided into five groups and there were 12 rats in each group, which including no any treated gestational diabetic model control group, resveratrol（60, 120 or 240 mg/ kg） treated groups and Metformin hydrochloride 200 mg/kg treated group. Except that, 12 rats with gestation for five days into normal gestation control group and 12 non-pregnant rats into another control group. The level of plasma glu- cose and insulin were determined before the drugs treatment and 7, 14 days after treatment. After two weeks of treat- ment, the body weight was detected , the content of TC, TG, LDL C, HDL C in serum were detected, and the level of Leptin, Adiponectin, Chemerin, TNFa, IL6 in plasma were also detected. Results： Compared with normal pregnant rats, the level of plasma glucose of rats and the body weight of rats in gestational diabetic model group were signifi- cantly increased and the level of insulin was significantly decreased;the level of TC, TG, LDL C were significantly in- creased and the HDL C was significantly decreased; the level of Leptin, Chemerin, TNFα, IL6 in plasma were significantly increased and the Adiponectin was significantly decreased. Compared with the rats with gestational diabetic in no resveratrol treated group, the content of TC and TG, the level of plasma glucose, the content of LDL C, the lev- el of Leptin, Chemerin, TNFa, IL6 in plasma and the body weight of rats in resveratrol treated groups were signifi cantly decreased, but the level of insulin, the content of HDL C and the level of Adiponectin in plasma were signifi- cantly increased. And all of above changes complied with dose dependent of resveratrol. Compared with the Metformin hydrochloride treated group, the level of insulin and

关 键 词：白藜芦醇 妊娠期糖尿病 血脂 脂肪因子 脂代谢 

分 类 号：R714.256[医药卫生—妇产科学]