白藜芦醇对β淀粉肽_（1-42）刺激内皮细胞炎症因子表达的影响  

The effects of resveratrol on the express of inflammatory factors of human umbilical vein endothelial cells induced by Aβ_(1-42)

作　　者：李焱平[1] 刘礼富 胡高强[1] 张静[2] 姜鲜[2] 

机构地区：[1]四川省泸县人民医院检验科,646100 [2]泸州医学院附属医院麻醉科,四川泸州646000

出　　处：《重庆医学》2015年第32期4481-4482,共2页Chongqing medicine

基　　金：四川省科技厅-泸州医学院联合项目(14JC0181);泸州市科技局课题(2012s37)

摘　　要：目的探索白藜芦醇对β淀粉样蛋白1-42（Aβ1-42）刺激内皮细胞炎症因子IL-1、IL-6与TNF-α表达影响。方法5×101μmol/L Aβ1-42刺激人脐静脉内皮细胞（HUVEC）24h,同时给予白藜芦醇160、80、40、20μg/L干预,CCK-8法检测HUVEC细胞活性;ELISA法检测IL-1、IL-6和TNF-α水平。结果与模型组比较,白藜芦醇各浓度组可以明显提高Aβ1-42刺激后HUVEC细胞活性,抑制Aβ1-42致HUVEC的IL-1、IL-6与TNF-α表达,差异有统计学意义（P〈0.05）。结论 20μg/L以上浓度白藜芦醇可减少Aβ1-42致HUVEC炎症因子IL-1、IL-6与TNF-α的表达。Objective To explore the effects of resveratrol on the express of inflammatory factors（IL-1,IL-6,TNF-α）of human umbilical vein endothelial cells（HUVEC）induced by Aβ1-42.Methods HUVEC were stimulated with Aβ1-425×101μmol/L,last 24 hand administrated with resveratrol 160,80,40,20μg/L,HUVEC viability were detected by CCK-8;the concentration of IL-1,IL-6and TNF-αwere detected by ELISA.Results Compared with model group,160,80,40,20μg/L resveratrol could increase HUVEC survival rate,reduced HUVEC damage by Aβ1-42,inhibited the concentration of IL-1,IL-6and TNF-α（P〈0.05）.Conclusion Resveratrol over 20μg/L could reduce the release of IL-1,IL-6and TNF-αof HUVEC inducing by Aβ1-42.

关 键 词：内皮细胞 白藜芦醇 β淀粉样肽1-42 炎症因子 

分 类 号：R96[医药卫生—药理学]