基于特异性PCR和荧光检测技术快速鉴定艾纳香  

Rapid Identification of Blumea balsamifera by Specific PCR and Fluorescence Detection

作　　者：赵丹[1] 周涛[1] 袁媛[2] 肖承鸿[1] 江维克[1] 康传志 

机构地区：[1]贵阳中医学院,贵阳550025 [2]中国中医科学院中药资源中心,道地药材国家重点实验室,北京100700

出　　处：《植物研究》2015年第6期952-956,共5页Bulletin of Botanical Research

基　　金：贵州省中药现代化科技产业研究开发专项(黔科合ZY字[2013]3001号);贵州省教育厅普通高等学校特色重点实验室建设(黔教合KY字[2013]108号);贵阳中医学院研究生教育创新计划项目(ZYYCX12029)

摘　　要：为建立快速准确的艾纳香分子鉴定方法。采取筛选艾纳香及其混伪品基因组DNA的提取方法,针对艾纳香特异性位点设计引物,优化PCR扩增条件,荧光检测扩增产物。结果表明碱裂解法更适于艾纳香基因组DNA的提取;叶绿体基因(t DNA)特异引物能特异性扩增艾纳香DNA,其扩增产物荧光检测呈绿色,混伪品无反应发生。试验结果显示该法简化了分子鉴定过程,省时节力,且结果准确可靠,可作为艾纳香植物和药材的鉴定方法。The experiment was conducted to establish a rapid and reliable molecular identification method for Blumea balsamifera DC. By genomic DNA extraction method, we screened B. balsamifera and its adulterants, designed primers with the specific loci of B. balsamifera, and optimized the amplification conditions of PCR. We detected the PCR products by fluorescence reaction. The alkaline lysis method of extracting genomic DNA was more appropriate for B. balsamifera. It could specifically amplified by primers of tDNA, and its products were green fluorescent by fluorescence detection. While, its adulterants were non-reaction occurring. This method simplified operation steps and saved time with accurate result, and it could be served as identification method for B. balsamifera.

关 键 词：艾纳香 特异性PCR 荧光检测 

分 类 号：Q949.783.5[生物学—植物学]