SMART技术构建的辣椒疫霉菌与辣椒互作AD-cDNA文库  

Construction of AD-c DNA Library for Interaction of Phytophthora capsici and Pepper in SMART Technology

作　　者：周志国[1] 明月梅[1] 

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000

出　　处：《中国蔬菜》2015年第11期51-54,共4页China Vegetables

基　　金：河北省科技计划项目(12226306);河北省高校食药用菌应用技术研发中心项目(YF201411)

摘　　要：为了分离和克隆辣椒中疫霉菌诱导基因,以接种辣椒疫霉菌的叶片为材料,利用SMART技术构建辣椒疫霉菌与辣椒互作的双杂交c DNA文库。结果表明：该文库容量为3.6×106cfu,重组率88%左右,插入片段集中在300～2000bp之间,平均长度约为800bp,表明获得的文库质量较高,该文库将为分子育种提供重要的基因资源。In order to identify the Phytophthora capsici stress induced genes of pepper,a double-hybrid eDNA library of Phytophthora capsici infected pepper leaves was constructed using SMART technology.The results showed that the eDNA library contained 3.6 × 10～6 independent clones,and the recombination rate was 88%.Insert fragments ranged from 300 bp to 2 000 bp,and the average length of the library was about 800 bp.These data demonstrate that the library is qualified,and able to provide important genetic resources for molecular breeding.

关 键 词：辣椒疫霉菌 SMART技术 双杂交c DNA文库 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]