甲状腺肿瘤患者的MHC基因频率分布及sMICA表达水平初探  

Primary study on the MHC gene frequency distribution and s MICA expression levels in thyroid tumor

作　　者：黄颖烽[1] 沈樑[1] 熊白玉 丁浩强[2] 谭茵[3] 叶欣[2] 罗宏图[1] 梁柳森[1] 李小文[1] 区栋材 

机构地区：[1]广州医科大学附属广州市第一人民医院普外科,广东广州510180 [2]广州血液中心 [3]广州医科大学基础医学院

出　　处：《中国输血杂志》2015年第10期1204-1208,共5页Chinese Journal of Blood Transfusion

基　　金：广州市医药卫生科技重点项目(20121A021008);广东省社会发展领域科技计划项目(20120318036);广东省自然科学基金项目(9151006001000009)

摘　　要：目的初步研究甲状腺肿瘤患者MHC基因频率分布及s MICA表达水平及相互关系。方法提取2个甲状腺肿瘤病例组(44名经术后病理证实为甲状腺癌的患者及87名甲状腺良性肿瘤患者)、1个健康对照组(133名健康体检者)的全血基因组DNA,采用直接测序法做HLA、MICA、MICB高分辨分型;运用Pypop Win32分析各基因位点的基因频率分析;SPSS 17.0分析甲状腺癌患者与MICA/B及HLA等位基因的相关性。运用ELISA法对上述研究对象的血清做s MICA半定量检测。结果 MICB*004∶01在甲状腺癌组、甲状腺良性肿瘤组和健康体检组的频率分别为:1.351%(1/44)、5.769%(6/87)、10.078%(26/133),是免患甲状腺癌患者的保护等位基因[P=0.014,RR(95%CI)=0.122(0.016-0.91)];DRB1*15∶01和DRB1*04∶03为甲状腺良性肿瘤的保护等位基因(P<0.05,RR<1);甲状腺癌患者与甲状腺良性肿瘤及健康体检者血清s MICA水平(ng/m L)分别为:0.233 1±0.244 5 vs 0.1819±0.1947 vs 0.2284±0.2787(P>0.05)。结论 HLA基因与甲状腺肿瘤疾病存在关联,其中MICB*004∶01与患甲状腺癌风险呈负相关。Objective To primarily investigate the genetic immunological background in thyroid tumor patients and asso- ciate thyroid carcinoma with MICA gene expression level in patients sera. Methods Genomic DNA were extracted from two teams with thyroid tumors （44 patients with thyroid carcinoma , 87 with benign thyroid nodules） and one team of healthy controls （133 healthy population through physical examination）. The HLA, MICA and MICB alleles were identifie.d：; by SBT. The gene frequency was analyzed by PyPopWin32 software. SPSS 17.0 were used to analyze the association of cancer with MICA/B and HLA gene frequency. ELISA was applied to test the soluble MICA（sMICA） concentration in sera. Results The MICB＊ 004：01 frequencies in thyroid carcinoma, benign thyroid nodules and healthy population were 1. 351%, 5. 769% and 10. 078%, respectively. MICB＊ 004：01 was the protective factor of thyroid carcinoma （P = 0. 014, RR [ 95% CI ] = 0. 12210. 016 -0.91 ] ）. HLA-DRB1 ＊ 15：01 and DRB1 ＊ 04：03 were the protect I＇ve alleles in benign thyroid nodules （ P 〈 0. 05 ,RR 〈 1 ）. The level of sMICA among thyroid carcinoma, benign thyroid nodules and healthy population was （0. 2331±0. 2445） vs （0. 1819±0. 1947） vs （0. 2284±0.2787） ng/ml, respectively. Conduslon HLA system is associated with thyroid tumors, of which MICB＊004：01 is negatively correlated with thyroid carcinoma.

关 键 词：甲状腺癌 主要组织相容性复合物 HLA等位基因 主要组织相容性复合物Ⅰ链相关蛋白A 高分辨分型 

分 类 号：R457.1[医药卫生—治疗学] R736.1[医药卫生—临床医学]