电针预处理对脑缺血再灌注损伤小鼠海马神经元自噬的影响  

Effect of electroacupuncture preconditioning on autophagy in hippocampal neurons of mice with cerebral ischemia-reperfusion injury

作　　者：王丽[1] 陈怀龙[1] 张云甫[2] 马福国[1] 王明山[1] 冉强强 毕燕琳[1] 时飞[1] 

机构地区：[1]青岛大学附属青岛市市立医院麻醉科,266071 [2]济南市第五人民医院麻醉科

出　　处：《中华麻醉学杂志》2015年第8期935-938,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81273821）

摘　　要：目的 评价电针预处理对脑缺血再灌注损伤小鼠海马神经元自噬的影响.方法 健康雄性C57BL/6小鼠36只,10～ 12周龄,体重20～ 25 g,采用随机数字表法分为3组（n=12）：假手术组（S组）、脑缺血再灌注组（I/R组）和电针预处理组（EA组）.采用双侧颈总动脉阻闭法制备小鼠全脑缺血再灌注模型,脑缺血15 min后恢复灌注.EA组于缺血前5d开始接受电针预处理百会穴30 min,1次/d,连续5d,最后1次预处理后24 h制备模型.于再灌注24 h时行神经行为学评分,随后处死小鼠,取脑组织,HE染色,计算海马CA1区神经元损伤率,采用Western blot法检测海马LC3Ⅱ、Beclin-1和Bcl-2的表达水平.结果 与S组比较,I/R组和EA组神经行为学评分降低,神经元损伤率增加,LC3Ⅱ表达上调,Bcl-2表达下调,I/R组Beclin-1表达上调（P＜0.05）,EA组Beclin-1表达差异无统计学意义（P＞0.05）;与I/R组比较,EA组神经行为学评分升高,神经元损伤率减少,LC3Ⅱ和Beclin-1表达下调,Bcl-2表达上调（P＜0.05）.结论 电针预处理通过抑制海马神经元自噬减轻小鼠脑缺血再灌注损伤.Objective To evaluate the effect of electroacupuncture preconditioning on autophagy in hippocampal neurons of mice with cerebral ischemia-reperfusion （I/R） injury.Methods Thirty-six healthy male C57BL/6 mice, aged 10-12 weeks, weighing 20-25 g, were randomly assigned into 3 groups （n=12 each） using a random number table： sham operation group （group S）, I/R group and electroacupuncture preconditioning group （group EA）.Global cerebral I/R was induced by occlusion of bilateral common carotid arteries for 15 min followed by reperfusion in mice anesthetized with chloral hydrate.In group EA, electroacupuncture of Baihui acupoints lasting for 30 min was performed once a day for 5 consecutive days starting from 5 days before ischemia, and the model was established at 24 h after the last preconditioning.Neurological function was assessed and scored at 24 h of reperfusion.The mice were then sacrificed, and brains were removed and cut into sections which were stained with haematoxylin and eosin.The rate of injured neurons in the hippocampal CA1 region was calculated.The expression of LC3 Ⅱ , Beclin-1 and Bcl-2 in hippocampal tissues was assessed by Western blot.Results Compared with group S, the neurological scores were significantly decreased, the rate of injured neurons was increased, and LC3 Ⅱ expression was up-regulated, and Bcl-2 expression was down-regulated in I/R and EA groups, Beclin-1 expression was upregulated in group I/R, and no significant change was found in Beclin-1 expression in group EA.Compared with group S, the neurological scores were significantly increased, the rate of injured neurons was decreased, LC3 Ⅱ expression was down-regulated, and Bcl-2 expression was up-regulated in group EA.Conclusion Electroacupuncture preconditioning reduces cerebral I/R injury through inhibiting autophagy in hippocampal neurons of mice.

关 键 词：电刺激疗法 缺血预处理 海马 再灌注损伤 自噬 神经元 

分 类 号：R245[医药卫生—针灸推拿学]