脊髓IL-12在大鼠关节炎性痛维持中的作用  

Role of interleukin-12 in spinal cord in maintenance of arthritic pain in rats

作　　者：周亚兰[1] 李号令 李卉[2] 熊源长[1] 张玉秋[2] 许华[1] 

机构地区：[1]第二军医大学附属长海医院麻醉科,上海市200433 [2]复旦大学神经生物学研究所

出　　处：《中华麻醉学杂志》2015年第8期976-978,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81070895）

摘　　要：目的 评价脊髓IL-12在大鼠关节炎性痛维持中的作用.方法 成年雄性SD大鼠,体重200～ 300 g,6～8周龄,采用随机数字表法,将其分为4组：正常对照组（C组,n=6）、关节炎性痛组（AP组,n=9）、磷酸盐缓冲液组（PBS组,n=6）和IL-12抗体组（n=6）.采用左踝关节腔内注射完全弗氏佐剂50μl的方法制备大鼠关节炎性痛模型.IL-12抗体组于造模后第9天鞘内注射20μl羊抗大鼠IL-12抗体1.50 μg,PBS组给予0.01 mol/L PBS 20μl.分别于造模前（基础状态）及造模后第9、10天时测定机械缩足反应阈（MWT）.于最后一次痛阈测定结束后处死大鼠,取L4-6节段脊髓,采用免疫荧光法测定脊髓背角IL-12表达,另外AP组还测定IL-12与星形胶质细胞标志物胶质纤维酸性蛋白共表达情况.结果 与C组比较,AP组、PBS组和IL-12抗体组造模后第9、10天时MWT降低,脊髓背角IL-12表达上调（P＜0.01）;与AP组比较,IL-12抗体组造模后第10天时MWT升高,脊髓背角IL-12表达下调（P＜0.05或0.01）,PBS组造模后第10天时MWT及脊髓背角IL-12表达差异无统计学意义（P＞0.05）.AP组脊髓背角IL-12与胶质纤维酸性蛋白共表达.结论 脊髓IL-12参与了大鼠关节炎性痛的维持.Objective To evaluate the role of interleukin-12 （IL-12） in the spinal cord in the maintenance of arthritic pain （AP） in rats.Methods Adult male Sprague-Dawley rats, aged 6-8 weeks,weighing 200-300 g, were randomly divided into 4 groups using a random number table： control group （group C, n=6）;AP group （n=9）;phosphate buffer solution （PBS） group （n=6）;IL-12 antibody group （n =6）.AP was induced by injecting 50 μl of complete Freund＆#39; s adjuvant into the ankle joint cavity of the left hindpaw of rats anesthetized with isoflurane.Goat anti-rat IL-12 antibody 1.50 μg （20 μl） was intrathecally injected on 9 days after establishment of the model in group IL-12 antibody, while 0.01 mol/L PBS （20 μl） was administered in group PBS.The mechanical paw withdrawal threshold to yon Frey filament stimulation （MWT） was measured before establishment of the model （baseline） and on 9 and 10 days after establishment of the model.The rats were sacrificed after the last measurement of pain threshold, and the L4 6 segments of the spinal cord were obtained for detection of IL-12 expression in the spinal dorsal horn （by immunofluorescence） , and the co-expression of IL-12 and glial fibrillary acidic protein （an astrocyte marker） was examined simultaneously in group AP.Results Compared with group C, the MWT was significantly decreased, and the expression of IL-12 was up-regulated on 9 and 10 days after establishment of the model in AP, PBS and IL-12 antibody groups.Compared with group AP, the MWT was significantly increased at 10 days after establishment of the model, and the expression of IL-12 was down-regulated in group IL-12 antibody, and no significant change was found in the MWT at 10 days after establishment of the model and expression of IL-12 in group PBS.IL-12 was co-expressed with glial fibrillary acidic protein in group AP.Conclusion IL-12 in the spinal cord is involved in the maintenance of AP in rats.

关 键 词：SPINAL CORD INTERLEUKIN-12 ARTHRITIS Pain 

分 类 号：R614[医药卫生—麻醉学]