盐酸戊乙奎醚对双重打击致大鼠急性肺损伤时细胞凋亡的影响  

Effect of penehyclidine hydrochloride on cell apoptosis during acute lung injury induced by ＆quot;two-hit＆quot; in rats

作　　者：吴晓静[1] 杨旭明[2] 梁辉[2] 宋学敏[2] 

机构地区：[1]武汉大学人民医院麻醉科,430060 [2]武汉大学中南医院麻醉科

出　　处：《中华麻醉学杂志》2015年第8期1007-1010,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金青年基金（81101449）

摘　　要：目的 评价盐酸戊乙奎醚对双重打击致大鼠急性肺损伤时细胞凋亡的影响.方法 SPF级健康雄性SD大鼠30只,体重240 ～ 270 g,8周龄,采用随机数字表法分为3组（n=10）：假手术组（Sham组）、胸部创伤-失血性休克致急性肺损伤组（ALI组）和盐酸戊乙奎醚组（PHC组）.ALI组和PHC组制备胸部创伤-失血性休克急性肺损伤模型,Sham组只分离股动、静脉,不制备胸部创伤-失血性休克急性肺损伤模型;PHC组于胸部创伤前1h腹腔注射盐酸戊乙奎醚2 mg/kg.于模型制备成功后8h时处死大鼠取肺组织.光镜和电镜下观察肺组织病理学结果,采用Western blot法检测Bax、Bcl-2和caspase-3的表达,采用ELISA法测定TNF-α含量,采用TUNEL法计数凋亡细胞,计算细胞凋亡指数.结果 与Sham组比较,ALI组和PHC组肺组织Bax、caspase-3、TNF-α水平和细胞凋亡指数升高,Bcl-2表达下调（P＜0.05）;与ALI组比较,PHC组肺组织Bax、caspase-3、TNF-α水平和细胞凋亡指数降低,Bcl-2表达上调（P＜0.05）.PHC组肺组织病理学损伤较ALI组明显减轻.结论 盐酸戊乙奎醚通过抑制细胞凋亡减轻双重打击致大鼠急性肺损伤.Objective To evaluate the effect of penehyclidine hydrochloride on cell apoptosis during acute lung injury （ALI） induced by ＆quot;two-hit＆quot; in rats.Methods Thirty male SPF Sprague-Dawley rats, aged 8 weeks, weighing 240-270 g, were randomly assigned into 3 groups （n =10 each） using a random number table： sham operation group （group Sham）, ALI induced by blunt chest trauma and hemorrhagic shock group （group ALI）, and penehyclidine hydrochloric group （group PHC）.In ALI and PHC groups, the rats were subjected to the combination of chest trauma and hemorrhage （mean arterial pressure 35-40 mmHg mm Hg, lasting for 60 min） to establish a model of ALI.In group PHC, penehyclidine hydrochloric 2 mg/kg was injected intraperitoneally at 1 h before blunt chest trauma.At 8 h after successful establishment of the model, the rats were sacrificed, and lungs were removed for examination of the pathologic changes and for determination of Bax, Bcl-2 and caspase-3 expression （using Western blot）, tumor necrosis factor-alpha （TNF-α） content （by enzyme-linked immunosorbent assay） , and cell apoptosis （by TUNEL）.Apoptotic index was calculated.Results Compared with group Sham, the levels of Bax, caspase-3 and TNF-α and apoptotic index were significantly increased, and Bcl-2 expression was down-regulated in ALI and PHC groups.Compared with group ALI, the levels of Bax, caspase-3 and TNF-α and apoptotic index were significantly decreased, and Bcl-2 expression was up-regulated in group PHC.The pathologic changes of lungs were significantly reduced in group PHC than in group ALI.Conclusion Penehyclidine hydrochloride mitigates ALI induced by ＆quot;two-hit＆quot; through inhibiting cell apoptosis in rats.

关 键 词：胆碱能拮抗剂 胸部损伤 休克 出血性 急性呼吸窘迫综合征 成人 细胞凋亡 

分 类 号：R614[医药卫生—麻醉学]