磷酸肌酸钠对力竭运动大鼠心肌线粒体解偶联蛋白2和能量代谢变化的影响  

Effects of creatine phosphate disodium on the uncoupling protein 2 in myocardial mitochondria and the energy metabolism in rats after exhaustive exercise

作　　者：李晓燕[1] 纪丽丽[2] 张红明[1] 孟可[2] 张国明[1] 韩淑芳[1] 

机构地区：[1]济南军区总医院心内科,济南250031 [2]辽宁医学院研究生院,辽宁锦州121001

出　　处：《解放军医学杂志》2015年第11期897-901,共5页Medical Journal of Chinese People's Liberation Army

基　　金：2014全军后勤科研重大项目子项目(AWS13C008)~~

摘　　要：目的探讨磷酸肌酸钠对一次力竭运动大鼠心肌线粒体能量代谢变化的影响,探讨磷酸肌酸钠对应激心肌的保护机制。方法雄性Wistar大鼠36只,随机分为对照组(n=12)、一次力竭组(n=12)、磷酸肌酸钠组(n=12)。实验结束后测定各组心肌ATP含量及血浆肾上腺素(E)、去甲肾上腺素(NE)、游离脂肪酸(FFA)浓度,并采用RT-PCR及Western blotting检测心肌线粒体解偶联蛋白2(UCP2)表达情况。结果与对照组相比,一次力竭组、磷酸肌酸钠组血浆FFA、E、NE浓度均增高(P<0.05),心肌ATP含量均降低。RT-PCR及Western blotting结果显示,一次力竭组、磷酸肌酸钠组UCP2 m RNA表达量分别较对照组增加64%、24%,UCP2蛋白表达量分别较对照组增加65%、26%。相关分析显示,心肌组织UCP2表达量与血浆FFA浓度呈显著正相关(r=0.98,P<0.01),血浆FFA浓度与血浆E、NE水平呈显著正相关(r=0.93,r=0.88,P<0.01)。结论力竭运动后心肌组织UCP2表达量显著上调,ATP合成减少,能量代谢障碍,可能是运动导致心源性猝死的机制之一。补充外源性磷酸肌酸钠可拮抗运动应激导致的UCP2表达升高,增加ATP储备,改善心肌能量代谢,可能对运动导致的心源性猝死预防有一定的积极作用。Objective To explore the effect of creatine phosphate disodium（CPDS） on the energy metabolism of myocardial mitochondria in rats after single exhaustive exercise, and the underline mechanism of protection effects of CPDS on myocardium under stress situation. Methods Thirty six male Wistar rats were randomly divided into control group, once exhaustive exercise group, and CPDS group（12 for each）. The concentrations of myocardial ATP, plasma epinephrine（E）, norepinephrine（NE） and free fatty acid（FFA） were determined. RT-PCR and Western blotting were performed to detect the expressions of uncoupling protein 2（UCP2） in myocardial mitochondria. Results The concentrations of plasma FFA, E and NE increased and the contents of myocardial ATP declined significantly in single exhaustive exercise group and CPDS group as compared with that in control group（P0.05）. RT-PCR revealed that the expressions of UCP2 m RNA were up-regulated by 64% and 24%, respectively, in single exhaustive exercise group and CPDS group when compared with that in control group. Western blotting showed that the expressions of UCP2 were increased by 65% and 26%, respectively, in single exhaustive exercise group and CPDS group when compared with that in control group. Correlation analysis showed a significant positive correlation of the expression of myocardial UCP2 to the concentration of plasma FFA（r=0.98, P0.01）, also of the concentration of plasma FFA to the levels of plasma E and NE（r=0.93, r=0.88, P0.01）. Conclusions After exhaustive exercise, the expression of myocardial UCP2 is significantly up-regulated, the synthesis of ATP reduced, and the energy metabolism disturbed, which may be one of the mechanisms leading to sudden cardiac death in sports training. Meanwhile, supplement of external CPDS may antagonize the increased expression of UCP2 induced by exercise stress, increase ATP reserves and improve the energy metabolism, and it may have a positive effect on prevention of sudden cardiac death induced by exha

关 键 词：解偶联蛋白2 猝死 心脏 运动过度 磷酸肌酸钠 能量代谢 

分 类 号：R541[医药卫生—心血管疾病]