二甲双胍对2型糖尿病模型大鼠足细胞podocalyxin表达的影响  

Effect of metformin on expression of podocalyxin in renal tissue of type 2 diabetes mellitus rats

作　　者：翟丽敏[1] 叶山东[1] 顾俊菲[1] 杨迪[1] 胡闻 

机构地区：[1]安徽医科大学附属省立医院内分泌科,合肥230001 [2]安徽医科大学附属省立医院病理科,合肥230001

出　　处：《中华老年心脑血管病杂志》2015年第11期1188-1192,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：安徽省自然科学基金(11040606M159);安徽高校省级自然科学研究项目(KJ2011A157)

摘　　要：目的观察不同剂量二甲双胍(MET)对高脂饮食联合低剂量链脲佐菌素诱导的2型糖尿病大鼠肾组织足细胞podocalyxin(PCX)表达的影响。方法选择SD大鼠48只,随机分为对照组(8只)、模型组(9只)、格列本脲组(8只),低剂量MET组(8只)、中剂量MET组(8只)和高剂量MET组(7只)。8周末测尿白蛋白、PCX和肌酐,并计算尿白蛋白/肌酐(UACR)、尿PCX/肌酐(UPCR)。取血测空腹血糖、糖化血红蛋白(HbA1c)、尿素、TG和LDL-C。留取肾组织做免疫组织化学及实时PCR检测PCX蛋白和mRNA表达,电镜观察肾组织病理改变。结果与对照组比较,模型组大鼠空腹血糖、HbA1c、尿素、TG、LDL-C、UACR、UPCR、基底膜厚度(GBMT)和足突融合率(FRFP)明显增加,而肾组织PCX蛋白和mRNA表达明显减少(P<0.05)。与模型组比较,不同剂量MET组尿素、TG、LDL-C、GBMT、FRFP、UACR和UPCR水平明显减低,肾组织PCX蛋白和mRNA表达呈剂量依赖性的增加(P<0.05)。与格列本脲组比较,不同剂量MET组血尿素、TG、LDL-C、GBMT、FRFP、UACR和UPCR水平明显减低,肾组织PCX蛋白和mRNA表达增加(P<0.05)。结论 MET可以通过调节2型糖尿病模型大鼠肾组织PCX的表达,发挥降糖以外的肾组织足细胞保护作用,且作用具有一定的剂量依赖性。Objective To study the effect of metformin（MET）on expression of podocalyxin（PCX）in renal tissue of type 2diabetes mellitus rats.Methods Forty eight SD rats were divided into control group（n=8）,model group（n=9）,glyburide group（n=8）,low MET dose group（n=8）,moderate MET dose group（n=8）and high MET dose group（n=7）.Urinary albumin（UA）,PCX and creatinine（CR）,and UA/CR ratio（UACR）,UPCX/CR ratio（UPCR）were assayed at the end of 8weeks.Serum levels of FPG,HbA1 c,urea,TG and LDL-C were measured.PCX protein and mRNA expression was detected by immunohistochemistry and RT-PCR respectively.Renal tissue lesions were observed under electron microscope.Results The levels of FPG,HbA1 c,urea,TG,LDL-C,and UACR,UPCR,basement membrane thickness（GBMT）and foot process fusion rate（FRFP）were significantly higher while the expression level of PCX protein and mRNA was significantly lower in model group than in control group（P〈0.05）.The levels of urea,TG,LDL-C,and GBMT,FRFP,UACR,UPCR were significantly lower while the expression level of PCX protein and mRNA was significantly higher in three MET dose groups than in model group and glyburide group（P〈0.05）.Conclusion MET protects renal podocytes by regulating PCX expression in renal tissue of type 2diabetes mellitus rats in a dose-dependent manner.

关 键 词：二甲双胍 链脲菌素 糖尿病 2型 血糖 RNA 信使 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]