用双波长紫外分光光度法测定尿囊素维E乳膏中维生素E的含量  

Determination of vitamin E in allantion vitamin E cream by dual wavelength UV-spectrophotometric method

作　　者：陆松伟[1] 虞慧华[1] 陈方剑[1] 宋洪杰[1] 

机构地区：[1]第二军医大学长海医院药学部,上海200433

出　　处：《药学服务与研究》2015年第5期354-356,共3页Pharmaceutical Care and Research

基　　金：军队医疗机构制剂标准提高科研专项课题-外用特色制剂质量标准提升研究(13ZJZ11-3)

摘　　要：目的：建立测定尿囊素维E乳膏中维生素E含量的双波长紫外分光光度法。方法：采用紫外一可见分光光度计测定维生素E的含量，测定波长为285nm，参比波长为293．2nm。结果：维生素E在31．96～95．88μg／ml范围内线性关系良好（r-O．9999），平均回收率为100．80％，RSD为1．21％（n=-9）。结论：该方法准确、灵敏，专属性强，重现性好，对尿囊素维E乳膏质量控制标准的提高具有参考意义。Objective： To establish a dual wavelength UV-spectrophotometric method for the determination of vitamin E in allantion vitamin E cream. Methods： The UV-visible spectrophotometer was used for content determination of vitamin E. The detection wavelength and reference wavelength were 285 nm and 293.2 nm,respectively. Results：The linear range of vitamin E was 31.96-95.88 μg/ml（r=0. 999 9）. The average recovery was 100.80G ,RSD was 1.21% （n= 9）. Conclusion： The method was more accurate, sensitive, specific and reproducible. It could provide the reference for the improvement of the quality stand- ards of allantion vitamin E cream.

关 键 词：维生素E 尿囊素维E乳膏 含量测定 分光光度法 紫外线 

分 类 号：R917[医药卫生—药物分析学]