胃酸分解三聚氰胺导致肾损伤及其自行修复的研究  

Gastric acid converts melamine causing kidney damage and its repair

作　　者：綦峥[1,2,3] 于淼[1,2,3] 辛国松[1,2,3] 季宇彬[1,2,3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心 [3]哈尔滨商业大学药物研究所博士后科研流动站

出　　处：《毒理学杂志》2015年第5期334-338,共5页Journal of Toxicology

基　　金：黑龙江省博士后落户基金(LBH-Q14093)

摘　　要：目的分析动物三聚氰胺染毒后三聚氰酸的来源及所致肾损伤的修复过程。方法 800 ng/ml的三聚氰胺溶液调节p H值分别为3、4、5和6 h后放置于37℃的恒温水域锅内酸化以模拟胃部条件,分别设定反应时间为1、2和3 h后,70℃氮气吹干后用气相色谱-质谱法检测反应终液中所含三聚氰胺和三聚氰酸浓度。78只Wistar雄性大鼠随机分成对照组与实验组,实验组60只,对照组18只。实验组动物染毒剂量为25 mg/kg,摻入饲料染毒5周。分别于染毒2周、3周和5周,及染毒停止后2周、6周和12周取实验组动物10只和对照组3只进行肾功能检测及肾脏组织病理学检查。结果三聚氰胺在体外模拟胃酸条件下可逐步分解成三聚氰酸,且分解量与p H值和反应时间呈线性关系。大鼠经三聚氰胺处理5周后BUN和CRE明显高于对照组动物,同时病理学也有明显变化。但在停止染毒过后不经任何干预处理,动物肾损伤可逐渐自行恢复。结论体外实验证明三聚氰胺至三聚氰酸在胃酸作用下的分解过程可以是纯化学反应;体内实验表明大鼠停止三聚氰胺染毒后,肾损伤可逐渐自行修复。Objective To investigate the formation of cyanuric acid and repair from kidney damage. Method GC-MS was conducted to analyze the concentration of melamine that was converted to cyanuric acid under simulate gastric condition. Melamine was mixed in the diet and administered at 25 mg/kg body weight to the exposure group. The rats were individually subjected to morphological observation after two, three, and five weeks of exposure, and two, six, and twelve weeks of recovery after exposure. Results Melamine can be converted to cyanuric acid under simulated gastric conditions. Renal tubules were obstructed by melamine-cyanuric acid crystals, but this condition can be gradually restored without further treatment after exposure to melamine was terminated. Conclusion The decomposition of melamine to cyanuric acid under gastric acid conditions is one of the mechanisms leading to kidney injury. Nevertheless, kidney damage can be gradually repaired without treatment.

关 键 词：三聚氰胺 三聚氰酸 胃酸 肾损伤 

分 类 号：R994.3[医药卫生—毒理学]