温度与放置时间对产前筛查标本的影响  

Effect of temperature and the duration on second-trimester serum markers

作　　者：单群达 黄珍珍 陈鹏龙 蓝海鹰 靳春雷 陈莉莉 徐雪琴 

机构地区：[1]浙江省丽水市妇幼保健院产前诊断中心,浙江丽水323000 [2]浙江省温州市中心医院中心实验室,浙江温州325000

出　　处：《中国现代医生》2015年第30期11-14,共4页China Modern Doctor

基　　金：浙江省人口计生委科研项目(JSW2012-A001)

摘　　要：目的探讨在产前筛查血清标本离心前放置时间及温度对游离人绒毛膜促性腺激素13亚基（free β—heg）、甲胎蛋白（AFP）、游离雌三醇（uE3）；N定结果的影响。方法额外收集产前筛查标本全血6mL。分装至5支试管（水浴组加做一支用于对照），在室温（23℃～25℃）或水浴箱（35℃～37℃）下分别放置0．5h、2h、4h,6h、8h后离心分离血清．以室温下0．5h分离血清的标本作为对照组，各组与对照组分别进行比较分析。结果置室温组样本freeB—heg以约每小时0．9％的速率上升，AFP、uE3与对照组比较差异无统计学意义（P〉0．05）；水浴条件下freeB—heg以约每小时10．9％的速率迅速升高，AFP以每小时0．6％的速率上升，uE3则以每小时0．5％的速率降低。结论室温条件下，2h内分离对结果影响较小，水浴条件对freeB—heg结果有严重影响，对其余两项也存在影响。产筛标本应尽量在较低温度下处理，建议不要对产前筛查标本进行37℃水浴处理。Objective To examine the effect of the duration of storage of whole blood at different controlled tempera- tures on the concentrations of serum free-B-human chorionic gonadotropin （f-βhCG）,Alpha fetoprotein（AFP） and uneon secrated estriol （uE3）. Methods We additionally collected 6 mL of whole blood of prenatal screeningspecimen, respec- tively put into 5 vacuum tubes（The water bath group should make an additional piece for comparison）, placed at room temperature （23℃-25℃） or in the water bath box （35℃-37℃） for 0.5 h, 2 h, 4 h, 6 h, 8 h, after that, made a centrifu- gal separation to separate the blood serum, taken the specimen of blood serum separated atroom temperature for 0.5 h as the control group, and respectively compared and analyzed each group with the control group. Results In the room temperature group, f-~hCG had a rate of 0.9% per hour increasing. No significant difference was found in AFP and uE3（P〉0.05）. In the 37℃ group, f-13hCG had a rate of 10.9% per hour increasing rapidly. AFP had a rate of 0.6% per hour increasing, and uE3 had a rate of 0.5% per hour decreasing. Conclusion Under the condition of room tempera- ture, to separate in 2 hours has a less influence on the result, and the water bath condition has a serious effect on the results of free 13-hcg and the other two items. The prenatal screening specimen should be processed at a lower tempera- ture as far as possible, and it is not suggested to make a water bath treatment of 37℃ for the prenatal screening Speci- men.

关 键 词：分析前变异 产前筛查 质量控制 温度 

分 类 号：R715.3[医药卫生—妇产科学]