MicroRNA218-Robo1通路对乳腺癌细胞迁移的抑制作用和机制的研究  

Rsearch of mechanism inhibitory effect on MicroRNA218-Robo1 pathway in breast cancer cell migration

作　　者：宋琼[1] 王其敏[1] 马婕妤[1] 孙鼐[1] 陈秋[1] 马艳玲[1] 李保林[1] 

机构地区：[1]郑州大学附属郑州中心医院麻醉科,郑州450007

出　　处：《国际外科学杂志》2015年第9期604-607,F0004,共5页International Journal of Surgery

基　　金：河南省科技厅科技发展计划项目（No.142102310459）

摘　　要：目的 明确MicroRNA218-Robo1通路在动物体内对乳腺癌的抑制功能及其机制.方法 BALB/c-nu/nu雌性裸小鼠40只,分为4组,每组动物分别用转染后高表达MicroRNA218的MDA-MB-231细胞、转染后共同高表达MicroRNA218和Robo1基因的MDA-MB-231细胞、转染后敲除Robo1基因的MDA-MB-231细胞以及阴性对照的MDA-MB-231细胞.从接种当天开始,每2周采用精诺真活体成像系统（IVIS 200）观测皮下肿瘤细胞的生长情况.TUNEL染色检测凋亡细胞,CD31染色检测肿瘤微血管密度,Ki-67染色检测肿瘤细胞增殖情况.结果 MicroRNA218组的肿瘤体积明显小于对照组,Robo1敲除组的肿瘤体积明显小于共同高表达MicroRNA218和Robo1组,差异有统计学意义;MicroRNA218和Robo1敲除组较对照组,乳腺癌细胞凋亡增多,细胞增殖和新生血管产生受到抑制.结论 MicroRNA218能够通过调节Robo1的表达抑制乳腺癌肿瘤细胞的迁移和侵袭.Objective To observe the migration and inhibition mechanism of MicroRNA218-Robo1 pathway for breast cancer.Methods A total of 40 BALB/c-nu/nu female mice were randomly divided into four groups.Each group was transfected over-expression MicroRNA218 MDA-MB-231 breast cancer cells, co-over-expression MicroRNA218 and Robo1 MDA-MB-231 breast cancer cells, knock-down Robo1 MDA-MB-231 breast cancer cells and the control MDA-MB-231 breast cancer cells.The tumor volume was examined every two weeks.Results Tumor volume of MicroRNA218 group was obviously less than control group, tumor volume of Robo1 knock out group was obviously less than common MicroRNA218 high expression and Robo1 group, the difference was statistically significant;MicroRNA218 and Robol knockout group than the control group, the increase in breast cancer cells apoptosis, cell proliferation and angiogenesis is restrained.Conclusions MicroRNA218 inhibited the migration of breast cancer by down-regulating the expression of Robo1.

关 键 词：乳腺肿瘤 MDA-MB-231细胞 活体成像 MICRORNA 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]