盐析法纯化新鲜蓝藻中藻蓝蛋白工艺条件的研究  

Research on Technology Conditions of Purifying Phycocyanin from Fresh Algae Using Salt Precipitation

作　　者：张发宇[1] 赵冰冰[1] 蔡静[2] 袁梦媛 盛晶梦 汪家权[1] 

机构地区：[1]合肥工业大学资源与环境工程学院,安徽合肥230009 [2]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《环境工程技术学报》2015年第6期499-503,共5页Journal of Environmental Engineering Technology

基　　金：国家"十二五"科技重大专项资助项目(2012ZX07103-004)

摘　　要：以水华暴发的新鲜蓝藻为处理对象,采取冻融破壁的方式获取藻蓝蛋白的粗提液。在一步盐析与二步盐析单因素试验的基础上,基于正交试验综合考虑盐析时间、藻蓝蛋白的浓度、p H和(NH4)2SO4饱和度对纯化藻蓝蛋白的影响。结果表明,一步盐析中最优工艺条件是,(NH4)2SO4饱和度为17%、藻蓝蛋白粗提液浓度为1.45 gL、p H为6.4、盐析时间为15 min;二步盐析中最优工艺条件是,(NH4)2SO4饱和度为35%、p H为4.0、盐析时间为15min。最终能得到纯度为2.04的藻蓝蛋白,有利于藻蓝蛋白的进一步纯化。The fresh blue-green algae in algae bloom were used for treatment and the crude extracts of phycocyanin were obtained by freeze-thaw method.On the basis of single-factor experiment of salt precipitation in the first and second steps, salt precipitation time, the impacts of phycocyanin concentration, pH and （ NH4 ） 2 SO4 saturation on the purifying phycocyanin were comprehensively considered using the orthogonal experiment.The results showed that the optimum conditions in the first step were 17% of （NH4）2SO4 saturation, 1.45 g/L of crude phycocyanin extract, pH 6.4 and 15 min of reaction time.In the second step, the optimum （NH4）2SO4 saturation was 35%, with pH 4.0 and 15 min of reaction time.The purity of phycocyanin was 2.04 at last, which would facilitate further purification.

关 键 词：盐析 纯化 藻蓝蛋白 正交试验 

分 类 号：X703[环境科学与工程—环境工程]