龙茭2号不同发育阶段茎部活性氧的检测分析  

Detection and Analysis of Active Oxygen in Different Developmental Stages of Longjiao 2

作　　者：康璐瑶 崔海峰[1] 邢阿宝 李帅[1] 叶子弘[1] 

机构地区：[1]中国计量学院生命科学学院/浙江省生物计量与检验检疫技术重点实验室,杭州310018

出　　处：《长江蔬菜》2015年第22期92-94,共3页Journal of Changjiang Vegetables

基　　金：国家科技支撑计划项目(2012BAD27B01);国家自然科学基金项目(1201499);浙江省公益项目(2014C32022)

摘　　要：基于龙茭2号茎部不同发育阶段及不同部位相关保护酶活性变化,比较分析了茭白茎部发育过程中活性氧的变化。龙茭2号茎部横切面及纵切面的NBT染色观察发现:超氧化物歧化酶(SOD)主要分布在茭白茎部维管束周围,发育早期茎部尖端染色较深。SOD和过氧化物酶(POD)的活性检测分析发现,龙茭2号3个不同部位中SOD及POD的活性变化趋势一致,发育早期和后期保护酶活性较高,中期活性较低。In this study, changes of reactive oxygen in different developmental stages of Z. latifolia stem had been analyzed, based on the changes of the protective enzymes between different developmental stages and different parts of Longjiao 2. NBT staining of Longjiao 2 was scanned from longitudinal section to transverse section, we found that superoxide dismutase（SOD） mainly distributed around the vascular bundles of Z. latifolia stem. It was more deeply stained in the stem＇s tip at early development. By detecting the activity of SOD and peroxidase（POD） in Z. latifolia, we found that the changing of SOD and POD activity in three different parts of Longjiao 2 change tends to be consistent, the activity was higher in early and late developmental stage, but lower in medium stage.

关 键 词：茭白 活性氧 超氧化物歧化酶 过氧化物酶 

分 类 号：S645.2[农业科学—蔬菜学]