基于荧光分析法的可降解微针药物传输性能评估  

Drug delivery performance evaluation of dissolving microneedle based on fluorescence analysis method

作　　者：张鸣天[1] 姚水洪[1] 刘冉[2] 

机构地区：[1]北京化工大学管理科学与工程系,北京100029 [2]清华大学生物医学工程系,北京100084

出　　处：《中国医学物理学杂志》2015年第6期768-771,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(81471749);国家重大科学仪器专项(2011YQ030134);清华大学自主研究计划(20131089190);北京市生物医学技术与仪器实验室项目

摘　　要：目的:经皮给药是一种热点的递送方式,目前由可降解材料构成经皮微针芯片使这种技术向临床应用更近一步。可降解材料微针芯片在动物皮肤上的刺入及溶解情况直接反映了药物递送的效率,因此对微针在体内溶解情况和药物释放情况的研究十分重要。方法:文章利用荧光分析法实现对刺入小鼠体内微针的降解情况进行观测,同时对其药物传输性能进行评估。结果:对不同长度的可生物降解微针刺入小鼠前后进行对比,并得到其释放率。结论:长度为800μm的微针在小鼠体内释放率比500μm高,并证明了丝素蛋白微针良好的可生物降解性。Objective Transdermal drug delivery is a hot way of delivery. And the recent percutaneous microneedle chips made of biodegradable material make this technique closer to clinical application. The insertion and dissolution situation of the dissolving microneedle chip in animal skin directly reflect the efficiency of drug delivery, which is important for researching on the situation of microneedle dissolution and drug-release in vivo. Methods Fluorescence analysis method was used to observe the dissolution situation of dissolving microneedle inserted into mice, and to evaluate its delivery efficiency. Results The release rates before and after dissolving microneedles with different lengths inserted into mice were evaluated and compared. Conclusion The release rate in vivo of 800 μm microneedle is higher than that of 500 μm microneedle, which proves the silk fibroin microneedle has a good biodegradability.

关 键 词：丝素蛋白微针 生物降解 荧光分析法 药物传输 

分 类 号：TH789[机械工程—仪器科学与技术] R-331[机械工程—精密仪器及机械]