正极性聚丙烯驻极体对糖尿病大鼠皮肤结构的影响  

Effects of positive electrets on skin structure of diabetic rats

作　　者：苑旺[1] 梁媛媛[2] 崔黎丽[1] 江键[2] 王美玲[2] 石岩[3] 

机构地区：[1]第二军医大学药学院无机化学教研室,上海200433 [2]第二军医大学基础部数理教研室,上海200433 [3]第二军医大学海军医学系,上海200433

出　　处：《中国医学物理学杂志》2015年第6期835-840,共6页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(51477175)

摘　　要：目的:研究正极性驻极体作用在大鼠皮肤两侧形成静电场的稳定性,以及对糖尿病大鼠皮肤结构的影响,进而明确驻极体在经皮给药系统中对药物的促渗机制。方法:利用栅控恒压电晕充电系统制备不同表面电位的正极性聚丙烯驻极体。以糖尿病大鼠皮肤为研究对象,正常大鼠皮肤为对照,利用等温表面电位衰减测量大鼠皮肤两侧静电场的稳定性,利用光镜研究驻极体对糖尿病大鼠皮肤显微结构的影响。结果:(1)+1500 V驻极体作用糖尿病大鼠皮肤24 h,透过大鼠皮肤的等效表面电位可达驻极体初始表面电位的(61.78±2.38)%,且初始表面电位随时间衰减缓慢,表明大鼠皮肤处于较稳定的静电场中;(2)随着驻极体作用时间的延长,糖尿病大鼠皮肤角质层逐渐增厚,表皮层和真皮层细胞排列有序性增加,但是与正常大鼠皮肤相比,仍比较松散。结论:(1)正极性驻极体在糖尿病大鼠皮肤两侧形成了一定强度且稳定的静电场;(2)正极性驻极体产生的静电场,可以一定程度地改善大鼠皮肤的显微结构,而且角质层和表皮层细胞排列仍然松散,有利于大分子药物的经皮转运。Objective To discuss the medicine absorption-promoting mechanism of positive electrets in transdermal administration system by studying on the stability of the electrostatic field caused by the influences of positive electrets on both sides of rat skin, and the effects on skin structure of diabetic rats. Methods The positive polypropylene electrets with different surface potential were prepared by grid-controlled constant corona charge system. The skins of diabetic rats were treated as study objects, and the skins of normal rats were used as control group. The stability of the electrostatic field on both sides of the rat skin was measured based on isothermal surface potential decay, and the effects on skin microscopic structure of diabetic rats were analyzed by optical microscopy. Results After electrets of＋1500 V acted on rat skin for 24 h, electrets could penetrate through the equivalent surface potential of rat skin and achieve （61.78-4-2.38） % of the electrets initial surface potential. And the initial surface potential decayed slowly with time, which showed that the rat skin was in a stable electrostatic field. With the prolonged action time of electrets, the stratum comeum of diabetic rat skin was gradually thickener, and the cells of epidermis and dermis were more orderly, however, the cell arrangement of diabetic rat skin was still looser than that of the normal rat skin. Conclusion Positive electrets create a stable electrostatic field on both sides of the diabetic rat skin. The created electrostatic field improves the microscopic structure of the diabetic rat skin, and the stratum corneum and epidermis cells of diabetic rats still arrange loosely, making transdermal administration of macromolecular medicine possible.

关 键 词：驻极体 静电场 糖尿病 大鼠 皮肤 显微结构 经皮给药 

分 类 号：R312[医药卫生—基础医学] R587.1