正交试验优选马鞭草中总三萜酸的提取工艺  

Optimization of Extraction Technology of Total Triterpenoids from Verbena officinalis L. by Orthogonal Test

作　　者：陈改敏[1] 龚如东[1] 张迪[1] 

机构地区：[1]南阳医学高等专科学校,河南南阳473061

出　　处：《食品研究与开发》2015年第17期67-69,共3页Food Research and Development

基　　金：南阳市科技局项目(2013GG056)

摘　　要：优选马鞭草中总三萜酸的提取工艺。采用紫外分光光度法测定总三萜酸含量;以总三萜酸得率为指标,采用单因素试验考察乙醇浓度、料液比、提取时间、提取次数的影响,在此基础上设计正交试验优选马鞭草中总三萜酸的提取工艺。最佳提取工艺为80%乙醇35倍量超声提取3次,每次30 min。在此条件下,总三萜酸得率21.91 mg/g。该工艺稳定可行,为马鞭草中三萜酸的开发利用提供参考。To optimize the extraction technology of total triterpenoids from Verbena officinalis L.. The content of total triterpenoids was determined by UV with oleanic as reference; Effects of concentration of extraction solvent, solid-liquid ratio, extraction time and extraction times by taking yield of total triterpenoids as the index, basis on this, extraction technology was optimized by orthogonal test. The optimum extraction technology were as follow： extracted 3 times with 35 times the amount of 80 % ethanol, 30 min each time. Under these conditions, yield of total triterpenoids from Verbena officinalis L. was 21.91 mg/g. This extraction process was stable and feasible, which provide the reference for the development and utilization of triterpenoids from Verbena officinalis.

关 键 词：马鞭草 总三萜酸 正交试验 

分 类 号：TQ464[化学工程—制药化工]