大孔树脂纯化洋甘菊中总黄酮的工艺条件研究  

Enrichment and Purification Technology for Total Flavonoids from Chamomile by Macroporous Resin

作　　者：陈丽春[1,2] 梁祥辉[1] 龚金炎[1] 

机构地区：[1]浙江科技学院浙江省农产品化学与生物加工技术重点实验室,浙江杭州310023 [2]浙江工商大学食品感官科学实验室,浙江杭州310035

出　　处：《食品研究与开发》2015年第17期113-116,共4页Food Research and Development

基　　金：浙江省科技计划项目(2012C22026);重点实验室开放基金(2013KF0901)

摘　　要：对大孔树脂纯化洋甘菊中总黄酮工艺条件进行优化研究。建立紫外-可见分光光度法测定洋甘菊中总黄酮方法;以吸附率、解吸率为评价指标,考察树脂类型、上样浓度、上样体积、洗脱浓度、洗脱体积对纯化工艺的影响。通过绘制静态吸附平衡曲线、泄露曲线和动态解吸曲线,综合评判确定最优工艺。结果表明:AB-8树脂对洋甘菊中总黄酮纯化效果较好,当上样质量浓度为1.8 mg/m L,上样体积流量为1 BV/h;洗脱剂用70%乙醇,体积流量为1.0 BV/h对洋甘菊中总黄酮的吸附率为62.5%、解吸率68%、回收率61%。经AB-8大孔树脂纯化洋甘菊中总黄酮提高25.3%,此方法稳定可靠,可用于洋甘菊总黄酮的工业纯化要求。The purification process of total flavonoids from chamomile on macroporous resins was studied in this research. An UV-vis spectrophotometry method to determine the content of total flavonoids in chamomile was established. The adsorption equilibrium capacities and desorption ratios were measured as the evaluation indexes, and the effects of the factors such as resin type, initial concentration, sample volume, elution concentration and elution volume were determined. Static adsorption isotherms, adsorption kinetic curves and adsorption breakthrough curves were measured and compared, and the optimal conditions of the purification process were obtained as follows： the AB-8 resin, initial concentration 1.8 mg/mL, adsorption flow rate 1 BV/h,desorption solvent 70 % ethanol, desorption flow rate 1.0 BV/h. At those conditions, the adsorption capacity ratio of the total flavonoids from chamomile was 62.5 %, the desorption ratio was 68 % and the recovery ratio was 61 %. The content of the total flavonoids from chamomile extract could be increased to 25.3 % through the purification process on macroporous resin. This method was stable and reliable, could meet the purification requirements of the total flavonoids from chamomile for industrial production.

关 键 词：洋甘菊 大孔树脂 总黄酮 分离纯化 

分 类 号：TQ464[化学工程—制药化工]