螺旋藻体外清除自由基的ESR研究  

Investigation with ESR on the Radical Scavenging Effect of Spirulina in Vitro

作　　者：赵淑锐[1] 郑美青[1] 吴英婷[1] 薛冰[1] 

机构地区：[1]首都医科大学医学实验与测试中心,北京100069

出　　处：《食品研究与开发》2015年第18期91-94,共4页Food Research and Development

摘　　要：利用DPPH、Fenton反应、SNAP、黄嘌呤氧化酶氧化黄嘌呤反应产生活性有机氮自由基、羟自由基(·OH)、一氧化氮(NO)自由基、超氧阴离子(·O2-)自由基,以电子顺磁共振法(ESR)研究了螺旋藻体外清除上述四种自由基的作用。该方法操作简单易行,结果直观。结果表明螺旋藻能有效清除上述4种自由基,而且随浓度的增加抗氧化能力也增加,浓度在40 mg/m L时对超氧阴离子和有机氮自由基的清除率达到了90%以上。Organic nitrogen radicals, hydroxyl radicals（·OH）, nitric oxide（NO） radicals, superoxide anion radicals（·O^2-） were produced by DPPH, Fenton reaction, SNAP, oxidation reaction of xanthine and xanthine oxidase. The antioxidant activities of spirulina in different concentrations were determined by the four radicals scavenging activity using electron paramagnetic resonance（ESR）Technology. The method was simple and easy to observe the results. Experimental results showed that spirulina have strong free radical scavenging capacity. The free radical scavenging capacity was increased by increasing concentrations. when the concentration in 40 mg/mL,the scavenging rates of Superoxide anion and organic nitrogen radicals reached more than 90 %.

关 键 词：螺旋藻 DPPH 羟自由基 一氧化氮自由基 超氧阴离子 电子顺磁共振 

分 类 号：R285[医药卫生—中药学]