猪传染性胃肠炎病毒流行株的分离鉴定及其S基因序列分析  

Isolation and identification of an epidemic strain of porcine transmissible gastroenteritis virus and the sequence analysis of its S gene

作　　者：樊雅婷[1,2] 张志[2] 吴发兴[2] 刘爽[2] 董雅琴[2] 邵卫星[2] 段纲[2] 王树双[2] 李晓成[2] 

机构地区：[1]云南农业大学,昆明650201 [2]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《黑龙江畜牧兽医》2015年第11期32-35,286,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：科技基础性工作专项(2012FY111000);青岛市民生计划项目(13-1-3-91-nsh)

摘　　要：为了研究仔猪腹泻的病原,试验采用了猪髋动脉内皮细胞（PIEC）对流行病学调查中检测到的3份猪传染性胃肠炎病毒（TGEV）阳性病料进行了培养、连续传代、PCR扩增、测序以及序列分析。结果表明：经过5代以后可以分离到1株出现典型细胞病变的病毒,其TCID50可达1×10-5.33/0.1 m L,经过多种病原PCR检测证实该病毒为TGEV,并命名为T04株;对其S基因进行测序和分析,可知T04与TGEV其他毒株的核苷酸同源性为96.7%-99.8%,氨基酸的同源性为96.0%-99.5%。说明该分离毒株为典型的TGEV毒株,且从进化树可以看出T04与FJ株的同源性最高。To identify the pathogen of diarrhea in piglet, a porcine iliac endothelium cell （PIEC） was used to culture the 3 positive pathological materials with porcine transmissible gastroenteritis virus （TGEV） detected in an epidemiological survey. And then the continuous passages, PCR amplification, sequencing, and sequence analysis were carried out in the test. The results showed that a strain of virus with typical cytopathic effect （CPE） was isolated after five passages, and the fifty percent tissue culture infective dose （TCID50） of the virus could reach 1 × 10^-5.33/0.1 mL. This virus was confirmed as TGEV by PCR detection a variety of pathogens and named as T04 strain. The T04 strain shared 96.7% to 99.8% nucleotide homology and 96.0% to 99.5% amino acid homology with the other strains of TGEV based on the sequence and analysis of its S gene. The results indicate that this isolate belongs to a typical strain of TGEV, and it can be seen from polygenetic tree that T04 strain has high homology with FJ strain.

关 键 词：猪传染性胃肠炎病毒 腹泻 分离鉴定 S基因 序列分析 

分 类 号：S828[农业科学—畜牧学] S855[农业科学—畜牧兽医]