No longer ‘blob-ology': Cryo-EM is getting into molecular details  

作　　者：ZHU HongTao ZHU Ping 

机构地区：[1]National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences

出　　处：《Science China(Life Sciences)》2015年第11期1154-1156,共3页中国科学（生命科学英文版）

摘　　要：The structural information of functionally important mac- romolecular assemblies is a key to molecular biology and is of great interest to structural biologists. Cryo-electron mi- croscopy （cryo-EM）, particularly the single particle analysis （SPA）, has been regarded as one of the three primary tech- niques for structure determination, together with X-ray crystallography and nuclear magnetic resonance （NMR）. For single particle cryo-EM, a tiny amount （~3 μL） of puri- fied biological samples at a concentration around 0.1-2 mg mL-1 are placed onto an EM grid, blotted with a filter paper and rapidly frozen into a thin layer of vitreous ice. The EM grid with frozen samples is then transferred to a transmis- sion electron microscope for imaging.The structural information of functionally important macromolecular assemblies is a key to molecular biology and is of great interest to structural biologists.Cryo-electron microscopy(cryo-EM),particularly the single particle analysis(SPA),has been regarded as one of the three primary tech-

关 键 词：分子生物学 冷冻电镜 透射电子显微镜 单颗粒分析 斑点 结构信息 X射线晶体学 生物学家 

分 类 号：Q-336[生物学]