类风湿关节炎患者外周血T淋巴细胞亚群CD4^+/CD8^+表达的意义  

Expression and significance of peripheral blood T-lymphocytes subsets CD4/CD8 in patients with rheumatoid arthritis

作　　者：梅序桥[1] 吴阿阳[1] 郑燕苹[1] 陈宏浦[2] 郑源海[1] 杨惠聪[1] 张佳林[2] 

机构地区：[1]福建医科大学附属漳州市医院检验科,福建漳州363000 [2]福建医科大学附属漳州市医院血液科,福建漳州363000

出　　处：《检验医学与临床》2015年第21期3144-3146,3149,共4页Laboratory Medicine and Clinic

基　　金：福建省卫生厅青年科研课题立项(2011-2-51)

摘　　要：目的探讨类风湿关节炎(RA)患者外周血T淋巴细胞亚群CD4+/CD8+的表达变化对疾病进展的关系。方法采用流式细胞术检测94例RA患者和22例健康对照者外周血T淋巴细胞亚群CD4+/CD8+。其中94例RA患者按RA疾病活动指数(DAS28)评分分为低值、中值、高值3组。结果与健康对照组比较,RA组外周血中CD3+CD4+T淋巴细胞的表达率明显升高(P<0.05),其中CD3+CD4+T淋巴细胞在DAS28低值组为(41.03±9.53)%,DAS28中值组为(42.16±7.08)%,DAS28高值组为(43.72±9.63)%;CD3+CD8+T淋巴细胞表达率明显降低(P<0.05),其中CD3+CD8+T淋巴细胞在DAS28低值组为(21.33±6.67)%,DAS28中值组为(21.39±7.36)%,DAS28高值组为(21.28±6.60)%。结论CD4+/CD8+比值与RA患者疾病进程相关,表明CD4+/CD8+失衡在RA的发病中发挥了重要作用。Objective To investigate the correlation between the change of peripheral blood T-lymphocyte subsets CD4/CD8 with the disease progress in the patients with rheumatoid arthritis（RA）.Methods The peripheral blood T-lymphocytes subsets CD4/CD8 in 94patients with RA and 22 healthy controls were examined by flow cytometry.94 cases of RA were divided into low value,median value and high value groups by RA disease activity index（DAS28）scores.Results Compared with the healthy controls,the expression rate of peripheral blood CD3^＋CD4^＋T cells in the RA group was increased significantly（P〈0.05）,in which CD3^＋CD4^＋T cells were（41.03±9.53）%in DAS28 low value group,（42.16±7.08）%in DAS28 median value group and（43.72±9.63）%in DAS28 high value group.The expression rate of CD3^＋CD8^＋T was significantly decreased（P〈0.05）,in which CD3^＋CD8^＋T cells were（21.33±6.67）%in DAS28 low value group,（21.39±7.36）%in DAS28 median value group and（21.28±6.60）%in DAS28 high value group.Conclusion CD4^＋/CD8^＋ratio is associated with RA progression,indicating that the CD4^＋/CD8^＋imbalances plays an important role in the pathogenesis of RA.

关 键 词：类风湿关节炎 流式细胞 T淋巴细胞亚群 

分 类 号：R135.12[医药卫生—劳动卫生]