HPLC法测定宜昌胡颓子根中槲皮素的含量  

Content Determination of Quercetin in Elaeagnus Henryi Root by HPLC

作　　者：杨林[1] 谢瑶[1] 孙志伟[1] 秦文[1] 

机构地区：[1]湖北三峡职业技术学院,湖北宜昌443000

出　　处：《中国医学创新》2015年第30期96-98,共3页Medical Innovation of China

基　　金：2012年湖北省教育厅科研项目(B20128302);2012年宜昌市科技研究与开发项目医疗卫生课题(A12301-58)

摘　　要：目的:建立高效液相色谱(HPLC)法测定宜昌胡颓子根中槲皮素的含量。方法:采用反相高效液相法测定宜昌胡颓子根中槲皮素含量的,色谱柱为Agilent Promosil C18(250 mm×4.6 mm,5μm),洗脱程序为梯度洗脱,流速为1 m L/min,检测波长为238 nm;柱温为30℃,进样量为10μL,观察该方法的适用性、精密度、稳定性及重复性等指标。结果:槲皮素样品在考查浓度范围内线性关系良好(r=0.9999);平均回收率为99.66%。HPLC法测定宜昌胡颓子根中槲皮素含量的适用性、精密度、稳定性及重复性均较好。结论:HPLC法简便、准确、重复性好,可用于宜昌胡颓子根中槲皮素的含量测定。Objective：To establish a HPLC method for the content determination of quercetin in elaeagnus henryi root.Method：RP-HPLC method was used to measure the content of quercetin in elaeagnus henryi root.The determination was performed on Agilent Promosil C18（250 mm×4.6 mm,5 μm）with gradient elution at the flow rate of 1.0 m L/min.The detection wavelength was 238 nm.The column temperature was set at 30 ℃.The injection volume was 10 μL.The indexes of this method such as applicability,accuracy,stability,repeatability and so on were observed.Result： The linear relation of the quercetin was fairly good（r=0.9999） and the average recovery was 99.66%.The applicability,accuracy,stability and repeatability of the HPLC method were all good.Conclusion：The HPLC method is simple,accurate and reproducible,which is suitable for the content determination of elaeagnus henryi root.

关 键 词：宜昌胡颓子 槲皮素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]