不同产地杜仲药材水提液高效液相色谱指纹图谱及其共有模式  

Establishment of Fingerprint and Mutual Mode of Aqueous Extracts of Eucommia ulmoides Oliv. from Different Regions by HPLC

作　　者：黄徐英[1] 陈玲[1] 程静[1] 宋红萍[1] 

机构地区：[1]武汉市普爱医院药剂科,武汉430033

出　　处：《医药导报》2015年第11期1497-1500,共4页Herald of Medicine

摘　　要：目的：采用高效液相色谱法建立不同产地杜仲药材水提液指纹图谱。方法采用 SinoChrom ODS-BP （250 mm×4.6 mm,5μm）色谱柱,乙腈-0.1％磷酸溶液为流动相进行梯度洗脱,流速为1.0 mL·min^－1,检测波长为230 nm,柱温25℃。结果确定14个共有峰,10批不同产地杜仲药材水提液的色谱指纹图谱整体相似度在0.592-0.986,并得出对照指纹图谱。结论该指纹图谱方法简便、重复性好,为有效地控制杜仲药材的内在质量提供科学依据。Objective To establish fingerprint and mutual mode of aqueous extracts of Eucommia ulmoides Oliv.from different regions by high performance liquid chromatography （ HPLC）. Methods The column of SinoChrom ODS-BP （250 mm×4.6 mm, 5 μm） was used.The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution.The flow rate was 1.0 mL·min^-1 , the detective wavelength was 230 nm, and the column temperature was 25 ℃ . Results The fingerprint consisted of 14 common peaks.The overall similarity for ten batches of Eucommia ulmoides Oliv.from different regions was 0.592-0.986.The standard fingerprint of Eucommia ulmoides Oliv.was established by HPLC. Conclusion This fingerprint method is simple and repeatable, and it provides a scientific basis for the quality control of Eucommia ulmoides Oliv.

关 键 词：杜仲 色谱法 高效液相 指纹图谱 共有模式 

分 类 号：R282.71[医药卫生—中药学] R927.1[医药卫生—中医学]