菌核病菌毒素对向日葵叶片生理生化的影响  

Effects of Sclerotinia sclerotiorum toxin on sunflower physiology and biochemistry

作　　者：刘佳[1,2] 张匀华[2] 孟庆林[2] 石凤梅[2] 马立功[2] 李易初[2] 左豫虎[1] 

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319 [2]黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨150086

出　　处：《中国油料作物学报》2015年第5期719-723,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家向日葵现代产业技术体系建设(CARS-16)

摘　　要：为了明确向日葵对菌核病菌毒素的生理生化抗性机制,测定了粗毒素液体浸根处理后,不同抗性向日葵品种丰葵杂1号(FKZ1)和7101的叶片几丁质酶、β-l,3-葡聚糖酶活性和丙二醛、可溶性糖、游离脯氨酸、木质素的含量及组织细胞膜透性的变化。毒素处理后,向日葵叶片几丁质酶和β-l,3-葡聚糖酶的活性,丙二醛、可溶性糖、游离脯氨酸、木质素的含量显著提高,以上几种物质除丙二醛外,抗病品种与感病品种相比升高的速度快,幅度大。同时毒素可显著破坏植株细胞膜透性,抗病品种细胞膜的耐损伤能力强于感病品种。研究表明几丁质酶和β-l,3-葡聚糖酶的活性,可溶性糖、游离脯氨酸、木质素含量和细胞膜的耐损伤能力与向日葵对菌核病的抗性呈正相关,丙二醛含量与向日葵对菌核病的抗性呈负相关。To better understand the physiology and biochemistry resistance mechanism of sunflower on Sclerotinia sclerotiorum toxin,changes of β- 1,3- glucanase and MDA,soluble sugar,free proline,lignin and cell membrane permeability of sunflower leaves were investigated. 2 sunflower varieties Fengkuiza 1（ FKZ1） and 7101 with different resistance were treated with crude toxin from S. sclerotiorum. The above indexes（ except MDA） were significantly changed after toxin treatment especially in resistant cultivar. Cell membrane permeability was significantly damaged by toxin. Resistant FKZ1 had higher ability of damage- resistance than 7101（ susceptible）. Results showed that sunflower resistance to S. sclerotiorum had positive correlation with chitinase,β- 1,3- glucanase activity,soluble sugar,free proline,lignin content and cell membrane permeability. MDA was negatively correlated with the resistance.

关 键 词：向日葵 菌核病 毒素 生理生化 

分 类 号：S435.655[农业科学—农业昆虫与害虫防治]