尼可地尔对体外培养犬心肌细胞凋亡的影响  

Effect of Nicorandil on Apoptosis of Cultured Dog Cardiomyocyte in Vitro

作　　者：黄正新[1] 王先宝[2] 

机构地区：[1]海南省农垦三亚医院心内科,海南三亚572000 [2]南方医科大学珠江医院心血管内科,广东广州510000

出　　处：《医学临床研究》2015年第10期1921-1924,共4页Journal of Clinical Research

摘　　要：【目的】探讨尼可地尔对体外培养的犬心肌细胞凋亡的影响。【方法】取成功离体培养的新生犬心肌细胞，将其随机分为对照组（A组）、过氧化氢组（B组）、尼可地尔＋过氧化氢组（C组）、尼可地尔＋5-羟基癸酸＋过氧化氢组（D组）；然后通过对一系列细胞凋亡标志物的检测，探讨尼可地尔是否具有抗细胞凋亡的作用。【结果】通过采用TUNEL染色标记、细胞色素C移位、Caspase-3活化等方法显示B组心肌细胞凋亡率明显高于C组和A组，且尼可地尔的这种抗细胞凋亡的作用可以被ATP敏感钾通道阻滞剂5-羟基癸酸所阻断。B组Caspase-3活性随时间延长而逐渐增强，与对照组相比差异具有统计学意义（P〈0．001）；与B组相比，C组加入尼可地尔预处理后能够明显持续抑制Caspase-3活性，差异具有统计学意义（P〈0．001）。【结论】尼可地尔具有直接抗离体培养的新生犬心肌细胞凋亡的效果，其机制与线粒体上ATP敏感钾通道开放密切相关。[Objective]To explore the effect of nicorandil on the apoptosis of cultured neonatal dog cardiomyocytes in vitro.[Methods] The cultured neonatal dog cardiomyocytes were randomly divided into four groups： control group（group A） ; hydrogen peroxide （H2O2 ） group （group B）;nicorandil plus H2O2group （group C） ; nicorandil plus 5-hydroxydecanoate （5-HD） and H2O2 group（group D）. Whether nicorandil can in- hibit the apoptosis of cardiomyocytes was evaluated directly by using a number of apoptotic markers.[Results] TUNEL staining, cytochrome C translocation and Caspase-3 activation showed the apoptosis rate of group B was significantly higher than that of group C and group A, and the nicorandil anti apoptotic effect could be blocked by ATP sensitive potassium channel blocker 5- hydroxy decanoic acid. The activity of Caspase 3 in group B increased gradually with time, and the difference compared with the control group was statistically significant （ P 〈0.001） ; Compared with group B , group C was treated with nicorandil pretreatment and can significantly inhibit caspase-3 activity, and the difference was statistically significant （P 〈0.001）[Conclusions]Nicorandil has a direct anti effect on the apoptosis of cultured neonatal dog eardiomyocytes in vitro. The mechanism is closely related of mitochondrial ATP sensitive potassium openchannel.

关 键 词：尼可地尔/药理学 肌细胞 心脏/药物作用 细胞 培养的 细胞凋亡 KATP通道 

分 类 号：R542.2[医药卫生—心血管疾病]