航天搭载怀牛膝离体快速繁殖的研究  

Rapid Propagation by Tissue Culture of Achyranthes bidentata Bl. After Spaceflight

作　　者：李萍[1,2] 赵喜亭[1,3] 李明军[1,3] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]郑州澍青医学高等专科学校,河南郑州450064 [3]河南省高校道地中药材保育及利用工程技术研究中心/绿色药材生物技术河南省工程实验室,河南新乡453007

出　　处：《核农学报》2015年第10期1917-1923,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家中医药管理局中医药行业科研专项子课题(201407005-08);河南省创新型科技人才队伍建设工程(C20130037);新乡市科技创新平台建设项目(CP1401)

摘　　要：为探求航天搭载怀牛膝离体快速繁殖的方法,在由航天搭载怀牛膝种子获得无菌试管苗的基础上,研究不同植物生长物质对试管苗生长的影响。结果表明,多芽体发生型利于航天搭载怀牛膝试管苗的快繁,丛生芽诱导的适宜培养基为MS+0.01 mg·L-1TDZ,丛生芽生长的适宜培养基为MS+0.1 mg·L-1GA3,试管苗壮苗生根的适宜培养基为MS+0.05mg·L-1NAA+1 mg·L-1PP333。本研究为选育怀牛膝优良空间诱变品种(系)的快繁及应用奠定了基础。To explore the method of rapid propagation of Achyranthes bidentata Bl. after spaceflight,axenic plantlets were nurtured from space-carried A. bidentata Bl. seeds firstly and then the effect of different plant growth substances on the plantlet growth has been studied. The results show that,the rapid propogation of the plantlets after spaceflight was facilitated by the growth of multi-bud body. The optimum media for the induction and growth of multiple shoots respectively were the MS supplemented with 0. 01 mg·L^- 1TDZ and MS supplemented with 0. 1 mg·L^- 1GA3,rooting medium for plantlets was the MS supplemented with 0. 05mg·L^- 1NAA and 1 mg·L^- 1PP333. This study laid the foundation for rapid propagation and application of high-quality space mutation A. bidentata Bl. varieties（ lines）breeding.

关 键 词：怀牛膝 航天搭载 试管苗 快速繁殖 多芽体 

分 类 号：S567.239[农业科学—中草药栽培]