阿托伐他汀对冠心病大鼠心肌细胞的凋亡及caspase-12表达的影响  

Atorvastatin on Apoptosis and Caspase-12 Expression in Coronary Artery Disease Rats

作　　者：再努热木.艾则孜 热娜古丽.艾则孜 阿得力艾山 阿不力克木[1] 王小英[1] 高冉冉[1] 艾斯卡尔.沙比提 

机构地区：[1]新疆医科大学第一附属医院急诊中心内科,新疆乌鲁木齐830054

出　　处：《现代生物医学进展》2015年第32期6238-6240,6289,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81202773)

摘　　要：目的:探讨阿托伐他汀对冠心病大鼠心肌细胞的凋亡及caspase-12表达的影响。方法:21只清洁剂SD大鼠经高脂饮食冠心病造模成功后随机分为空白组、对照组和阿托伐他汀组每组7只。空白组大鼠未经处理,对照组给予生理盐水10 m L/kg体重灌胃,1天一次连续4周;阿托伐他汀组大鼠给予阿托伐他汀10 mg/kg体重灌胃,1天一次连续4周。4周后处死大鼠并取大鼠心肌组织采用TUNEL法检测细胞凋亡情况,Western-blot法检测caspase-12表达情况。结果:三组大鼠心肌凋亡比较空白组、对照组及阿托伐他汀组大鼠细胞凋亡比例分别为(3.72±0.89)%、(3.84±1.01)%和(1.47±0.62)%,阿托伐他汀大鼠心肌细胞凋亡比例明显低于空白组和对照组(P<0.05);阿托伐他汀组大鼠心肌细胞Caspase-12表达水平显著低于空白组与对照组(P<0.05),而空白组与对照组比较无统计学差异(P>0.05)。结论:阿托伐他汀可能通过抑制大鼠心肌细胞caspase-12表达来阻断心肌细胞凋亡途径的激活。Objective： To evaluate the effect of atorvastatin on apoptosis and caspase-12 expression in coronary artery disease rats. Methods： 21 coronary artery disease rats SD rats were randomly divided into 3 groups （blank group, control group and atorvastatin group） with each 7 rats in each group. The blank group without any treatment, the control group with 10 mL/kg body weight qd for intragastric administration for 7 days, and rats in the atorvastatin group were treated with atorvastatin 10 mL/kg body weight qd for intragastric administration for 7 days. After 7 days the rats were to put to death. The cell apoptosis and caspase-12 expression were arrayed by TUNEL and Western-blot among the three groups （P〉0.05）. Results： The apoptosis rate were （3.72± 0.89） %, （3.84± 1.01） % and （1.4± 0.62） % for the blank, control and atorvastatin groups respectively which indicated that atorvastatin much lower than the blank and control groups （P〈0.05）; The caspase-12 expression in atorvastatin group was significant lower than the other two groups with statistical difference（P〈0.05）. Conclusion： The cell apoptosis was suppressed by atorvastatin through inhibiting the caspase-12 expression in coronary artery disease rats.

关 键 词：阿托伐他汀 冠心病 大鼠 凋亡 CASPASE-12 

分 类 号：Q95-3[生物学—动物学] R541.4[医药卫生—心血管疾病]