大鼠局灶性脑缺血模型评价方法间关联性分析  

Analysis of the correlation between three evaluation methods of rat models of focal cerebral ischemia / reperfusion

作　　者：崔煦然 刘钊[1] 张志斌[1] 钟菊迎[1] 

机构地区：[1]中国中医科学院医学实验中心分子生物实验室中国中医科学院医学实验中心国家重点实验室,北京100700

出　　处：《中国实验动物学报》2015年第5期506-508,512,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：中国中医科学院院自主课题(ZZ2012012)

摘　　要：目的分析评价线栓法制备大鼠大脑中动脉阻塞模型成功的三种方法之间的相关性,为评价该模型提供一种新的方法和标准。方法雄性SD大鼠共(30)只,随机分成假手术组(n=15)、模型组(n=15),参照Zea-Longa线栓法稍加改进制备局灶性脑缺血模型,于术前术后检测各组大鼠脑血流变化。以大鼠脑梗死面积率为金标准,分析该标准与脑血流变化之间的相关性。结果模型大鼠脑梗死面积率与脑血流变化量呈正相关,与行为学评分高低无关。结论大鼠脑血流量变化可作为评价局灶性脑缺血模型成功的标准。Objective To analyze the correlation between three evaluation methods of middle cerebral artery occlu -sion and focal cerebral ischemia , and to explore a new method and standard for the evaluation of the model .Method Thirty healthy adult male SD rats were randomly divided into a sham-operated group （ n=20 ） and model group .According to Zea-Longa procedure ,we established the disease model to detect the changes in cerebral blood flow before and after sur -gery.The resulted cerebral infarction area was taken as gold standards .Then we analyzed the correlation between the standards and the changes in cerebral blood flow .Results The changes of infarction area ratio were positively correlated with the changes of cerebral blood flow in the model rats .Conclusions The changes of rat cerebral blood flow can be used to evaluate whether the cerebral ischemia model is successful or not .

关 键 词：脑梗死面积率 行为学评分 脑血流量 大鼠 

分 类 号：Q95-33[生物学—动物学]