急性出血坏死性胰腺炎大鼠肠道免疫功能的改变  

Intestinal mucosal immune barrier injury of rats with acute hemorrhagic necrotic pancreatiti

作　　者：金英朝[1] 王海宽[1] 张弘超[1] 李小东[1] 刘大伟[1] 

机构地区：[1]黑龙江省医院普外一科,黑龙江哈尔滨150086

出　　处：《临床医药文献电子杂志》2015年第30期6166-6168,共3页Electronic Journal of Clinical Medical Literature

摘　　要：目的探讨分析急性出血坏死性胰腺炎(AHNP)大鼠肠道黏膜免疫功能的变化情况。方法选取成年的Wistar雄性大鼠,随机分成AHNP组、假手术组。分别于造模后24 h取标本检测(主要包括内毒素的含量、CD3、CD4及CD8的阳性T淋巴细胞的比例、盲肠内容物s Ig A的浓度,血清肿瘤坏死因子-α(TNF-α)的水平等。结果 AHNP组各时间段的门静脉血内毒素含量均有显著增加,回肠末段黏膜固有层的CD3+、CD4+T比例显著降低,盲肠内容物s Ig A浓度明显降低,肠内容物中s Ig A浓度和血浆ET浓度、血清TNF-α含量均呈显著的负相关。结论 AHNP大鼠早期存在的肠黏膜免疫功能的显著下降不仅有可能引起肠内的菌群异常和内毒素移位,亦可诱发AHNP感染。ObjectiveTo observe intestinal mucosal immune barrier relative factors TNF-α in serum, sIgA levels of intestinal juice, and CD3＋ and CD4＋ cell change of intestinal tissue in rats with acute hemorrhagic necrotic pancreatitis. To investigate the change of intestinal mucosal immune barrier injury in this model.Methods36 wistar rats were randomly divided into three groups： SAP model group（A group）, the sham operation control group （B group） （N = 18）. Abdominal aortic and Portal vein blood were collected, and stored in refrigerator for next assays. Intestinal juice were collected and stored in refrigerator for next assays. The intestinal tissue were ifxed in 4% neutral formaldehyde buffer. TNF-α concentration of blood and sIgA levels of intestinal juice were evaluated by ELISA method. Immunohistochemistry was adopted to detect intestinal tissue CD3＋ and CD4＋ expression. Endotoxin levels of portal vein blood was detected following the kit instruction.ResultsTesting the indicators after model building of each group,we have the following ifndings： In group A, Blood TNF-a and sIgA levels of intestinal juice are signiifcantly lower than that in the group B, while endotoxin levels of portal vein blood signiifcantly higher. The expression of CD3＋ and CD4＋ T immune cell are signiifcantly lower in group A.ConclusionThis study conifrmed the participation of the intestinal mucosal immune barrier function injury are important inlfammatory disorders in acute pancreatitis in rats with acute hemorrhagic necrotic pancreatitis.

关 键 词：急性出血坏死性胰腺炎 肠道免疫功能 内毒素 TNF-Α 

分 类 号：R657.51[医药卫生—外科学]