微型大白菜“娃娃菜”游离小孢子出胚率影响因素研究  

Effect of Frequency of Embryos on Microspore Culture in Miniature Chinese Cabbage 'Baby cabbage'

作　　者：王丽丽[1] 王鑫[1] 吴海东[1] 田云[1] 吕艳玲[1] 

机构地区：[1]辽宁省农业科学院蔬菜研究所,辽宁沈阳110161

出　　处：《辽宁农业科学》2015年第5期5-8,共4页Liaoning Agricultural Sciences

基　　金：辽宁省博士科研启动基金(20131054);辽宁省科学事业公益研究基金(2013002001)

摘　　要：为了创制新、奇、特大白菜优异DH系,丰富大白菜种质资源,试验以3个"娃娃菜"优良杂交种为试材进行小孢子培养,通过优化培养条件建立高效培养体系,对影响小孢子胚发生的主要因素研究,结果表明:不同基因型间的小孢子胚诱导率差异极显著;4℃低温预处理24 h对小孢子胚的发生具有促进作用;33℃条件热击48 h,胚诱导率达最大值;NLN-13+0.05 mg/L 6-BA+0.05 mg/L NAA为适宜的诱胚培养基。This study is to develop new,odd,excellent DH lines in Chinese Cabbage for rich in Germplasm Resources.Isolated microspore culture was employed in 7 hybrid varieties.An efficient culture system was es-tablished through the optimization of culture conditions.Results showed that embryoids and 335 regenerated plants were obtained.Genotypes had extremely significant effect on frequency of embryogenesis.Low tempera-ture pretreatment at 4 ℃ for 24 h couldenhance embryo frequencies.Temperature pretreatment at 33 ℃ for 48 h couldenhance embryo frequencies.NLN-13 ＋0.05 mg /L 6-BA ＋0.05 mg /L NAA could improve the fre-quency ofmicrospore embryogenesis.

关 键 词：微型大白菜 游离小孢子培养 胚状体 

分 类 号：Q813.1[生物学—生物工程]