有机巯基小分子生物荧光探针研究进展  

Research progress in organic fluorescent probes for biological small molecules containing thiol

作　　者：杨志广[1] 李运林[1] 刘增臣[1] 田丰收[1] 彭鹏[1] 

机构地区：[1]周口师范学院化学化工学院,周口466001

出　　处：《分析试验室》2015年第11期1358-1364,共7页Chinese Journal of Analysis Laboratory

基　　金：河南省高等学校重点科研项目计划(15A430055);周口师范学院校本项目(ZKNUB115201)资助

摘　　要：生命体内许多重要的巯基小分子,如半胱氨酸(Cysteine,Cys)、同型半胱氨酸(Homocysteine,Hcy)和还原谷胱甘肽(Glutathione,GSH)等,在维持生命体系中的氧化还原平衡发挥着重要作用。因此,定量检测和专一性识别巯基生物分子在生物医学研究中具有非常重要的意义。荧光分析法具有操作简便、灵敏度高、选择性好、实时检测以及对生物体损伤小等优点而受到广泛关注。双光子荧光探针技术相对于单光子荧光技术具有长波吸收,短波发射、高度的三维空间选择性、大的穿透深度、避免荧光漂白和光致毒以及降低组织自发荧光干扰等特点,在生命科学领域具有广阔的应用前景。介绍了有机单光子和双光子巯基小分子荧光探针的研究现状,同时展望了有机巯基小分子荧光探针未来的研究方向。Many important biological small molecules containing thiol,such as cysteine（ Cys） 、homocysteine（ Hcy） and glutathione（ GSH）,play a key role in maintaining the biological redox homeostasis. Therefore,the quantitative detection and specific discrimination of thiol-containing molecules in biological samples are of great importance in biomedical research. Fluorescence methods have been extensively studied and applied due to simple operation,high sensitivity and selectivity,real-time detection and little damage to organisms. The two-photon fluorescent probe technique has shown much broader application prospect in the field of life sciences compared to the one-photon fluorescent probe technique due to many advantages,such as absorbing light of longer wavelength with lower energy but emitting shorter wavelength,high three-dimensional spatial selectivity,large penetration depth,avoidance of photodamage and photobleaching and lower tissue auto-fluorescence,etc. The research status of organic one-photon and two-photon mercapto Biomolecules fluorescent probes were introduced,and the trends of organic fluorescent probes for mercapto biomolecules in the future were also prospected.

关 键 词：巯基 荧光探针 双光子 检测 展望 

分 类 号：O657[理学—分析化学] O622[理学—化学]